| Title |
New reagents for increasing ESI multiple charging of proteins and protein complexes
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, November 2011
|
| DOI | 10.1016/j.jasms.2009.09.014 |
| Pubmed ID | |
| Authors |
Shirley H. Lomeli, Ivory X. Peng, Sheng Yin, Rachel R. Ogorzalek Loo, Joseph A. Loo |
| Abstract |
The addition of m-nitrobenzyl alcohol (m-NBA) was shown previously (Lomeli et al., J. Am. Soc. Mass Spectrom. 2009, 20, 593-596) to enhance multiple charging of native proteins and noncovalent protein complexes in electrospray ionization (ESI) mass spectra. Additional new reagents have been found to "supercharge" proteins from nondenaturing solutions; several of these reagents are shown to be more effective than m-NBA for increasing positive charging. Using the myoglobin protein-protoporphyrin IX (heme) complex, the following reagents were shown to increase ESI charging: benzyl alcohol, m-nitroacetophenone, m-nitrobenzonitrile, o-NBA, m-NBA, p-NBA, m-nitrophenyl ethanol, sulfolane (tetramethylene sulfone), and m-(trifluoromethyl)-benzyl alcohol. Based on average charge state, sulfolane displayed a greater charge increase (61%) than m-NBA (21%) for myoglobin in aqueous solutions. The reagents that promote higher ESI charging appear to have low solution-phase basicities and relatively low gas-phase basicities, and are less volatile than water. Another feature of mass spectra from some of the active reagents is that adducts are present on higher charge states, suggesting that a mechanism by which proteins acquire additional charge involves direct interaction with the reagent, in addition to other factors such as surface tension and protein denaturation. |
Mendeley readers
The data shown below were compiled from readership statistics for 148 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 1% |
| United States | 1 | <1% |
| Germany | 1 | <1% |
| Switzerland | 1 | <1% |
| Unknown | 143 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 50 | 34% |
| Researcher | 23 | 16% |
| Student > Master | 18 | 12% |
| Student > Bachelor | 13 | 9% |
| Other | 8 | 5% |
| Other | 16 | 11% |
| Unknown | 20 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 83 | 56% |
| Agricultural and Biological Sciences | 19 | 13% |
| Biochemistry, Genetics and Molecular Biology | 14 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
| Unspecified | 1 | <1% |
| Other | 4 | 3% |
| Unknown | 23 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2021.
All research outputs
#5,482,691
of 25,498,750 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#558
of 3,850 outputs
Outputs of similar age
#42,415
of 245,909 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#27
of 172 outputs
Altmetric has tracked 25,498,750 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,850 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 245,909 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 172 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.