| Title |
Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing™
|
|---|---|
| Published in |
European Journal of Clinical Microbiology & Infectious Diseases, April 2008
|
| DOI | 10.1007/s10096-008-0514-6 |
| Pubmed ID | |
| Authors |
B. Saager, H. Rohde, B. S. Timmerbeil, G. Franke, W. Pothmann, J. Dahlke, S. Scherpe, I. Sobottka, P. Heisig, M. A. Horstkotte |
| Abstract |
In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in E. faecium as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 3% |
| Germany | 1 | 3% |
| Unknown | 27 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 21% |
| Researcher | 5 | 17% |
| Professor > Associate Professor | 4 | 14% |
| Other | 2 | 7% |
| Lecturer > Senior Lecturer | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 5 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 24% |
| Medicine and Dentistry | 6 | 21% |
| Immunology and Microbiology | 3 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Nursing and Health Professions | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 8 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#4,836,804
of 23,323,574 outputs
Outputs from European Journal of Clinical Microbiology & Infectious Diseases
#441
of 2,812 outputs
Outputs of similar age
#15,893
of 82,380 outputs
Outputs of similar age from European Journal of Clinical Microbiology & Infectious Diseases
#5
of 13 outputs
Altmetric has tracked 23,323,574 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,812 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one has done well, scoring higher than 79% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.