| Title |
The subpopulation of microglia expressing functional muscarinic acetylcholine receptors expands in stroke and Alzheimer’s disease
|
|---|---|
| Published in |
Brain Structure and Function, December 2014
|
| DOI | 10.1007/s00429-014-0962-y |
| Pubmed ID | |
| Authors |
Maria Pannell, Maria Almut Meier, Frank Szulzewsky, Vitali Matyash, Matthias Endres, Golo Kronenberg, Vincent Prinz, Sonia Waiczies, Susanne A. Wolf, Helmut Kettenmann |
| Abstract |
Microglia undergo a process of activation in pathology which is controlled by many factors including neurotransmitters. We found that a subpopulation (11 %) of freshly isolated adult microglia respond to the muscarinic acetylcholine receptor agonist carbachol with a Ca(2+) increase and a subpopulation of similar size (16 %) was observed by FACS analysis using an antibody against the M3 receptor subtype. The carbachol-sensitive population increased in microglia/brain macrophages isolated from tissue of mouse models for stroke (60 %) and Alzheimer's disease (25 %), but not for glioma and multiple sclerosis. Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-γ to around 60 %. This increase was sensitive to blockers of protein synthesis and correlated with an upregulation of the M3 receptor subtype and with an increased expression of MHC-I and MHC-II. Carbachol was a chemoattractant for microglia and decreased their phagocytic activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 89 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 27% |
| Student > Bachelor | 11 | 12% |
| Researcher | 9 | 10% |
| Student > Doctoral Student | 7 | 8% |
| Other | 6 | 7% |
| Other | 14 | 15% |
| Unknown | 19 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 36 | 40% |
| Agricultural and Biological Sciences | 8 | 9% |
| Biochemistry, Genetics and Molecular Biology | 5 | 5% |
| Medicine and Dentistry | 5 | 5% |
| Immunology and Microbiology | 3 | 3% |
| Other | 8 | 9% |
| Unknown | 26 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2015.
All research outputs
#21,697,638
of 24,217,893 outputs
Outputs from Brain Structure and Function
#1,524
of 1,725 outputs
Outputs of similar age
#307,529
of 361,460 outputs
Outputs of similar age from Brain Structure and Function
#37
of 44 outputs
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We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.