| Title |
Spexin in the half-smooth tongue sole (Cynoglossus semilaevis): molecular cloning, expression profiles, and physiological effects
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, February 2018
|
| DOI | 10.1007/s10695-018-0472-6 |
| Pubmed ID | |
| Authors |
Shengpeng Wang, Bin Wang, Songlin Chen |
| Abstract |
Spexin (SPX), a novel neuropeptide discovered by the bioinformatics approach, has been shown to exert pleiotropic functions in mammals. However, little information regarding the physiological role of SPX is available in teleosts. As a first step, we cloned the spexin gene from a flatfish, the half-smooth tongue sole. The open reading frame (ORF) of tongue sole spexin contained 363 nucleotides encoding a 120 amino acid (aa) preprohormone with a calculated molecular mass and isoelectric point of 14.06 kDa and 5.86, respectively. The tongue sole SPX precursor contained a 27 aa signal peptide and a 14 aa mature peptide flanked by two dibasic protein cleavage sites (RR and GRR). Tissue distribution analysis showed that spexin mRNA could be detected in various tissues, notably in the brain. In addition, fasting stimulated the hypothalamic expression of spexin mRNA. Intraperitoneal injection of SPX increased gnih and gnrh3 mRNA levels in the hypothalamus; however, SPX inhibited the pituitary expression of gh, fshβ, and gthα mRNAs. Overall, our results reveal the existence of a functional SPX in the tongue sole, which could represent an important factor in the neuroendocrine control of flatfish reproduction and growth, and the spexin mRNA expression is regulated by feeding status. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
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| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 22% |
| Student > Master | 4 | 17% |
| Student > Bachelor | 2 | 9% |
| Professor | 2 | 9% |
| Student > Doctoral Student | 2 | 9% |
| Other | 4 | 17% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 26% |
| Biochemistry, Genetics and Molecular Biology | 4 | 17% |
| Agricultural and Biological Sciences | 4 | 17% |
| Sports and Recreations | 1 | 4% |
| Business, Management and Accounting | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 7 | 30% |
Attention Score in Context
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#20,462,806
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#608
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Outputs of similar age from Fish Physiology and Biochemistry
#16
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