| Title |
High level accumulation of gamma linolenic acid (C18:3Δ6.9,12 cis) in transgenic safflower (Carthamus tinctorius) seeds
|
|---|---|
| Published in |
Transgenic Research, August 2011
|
| DOI | 10.1007/s11248-011-9543-5 |
| Pubmed ID | |
| Authors |
Cory L. Nykiforuk, Christine Shewmaker, Indra Harry, Olga P. Yurchenko, Mei Zhang, Catherine Reed, Gunamani S. Oinam, Steve Zaplachinski, Ana Fidantsef, Joseph G. Boothe, Maurice M. Moloney |
| Abstract |
Gamma linolenic acid (GLA; C18:3Δ6,9,12 cis), also known as γ-Linolenic acid, is an important essential fatty acid precursor for the synthesis of very long chain polyunsaturated fatty acids and important pathways involved in human health. GLA is synthesized from linoleic acid (LA; C18:2Δ9,12 cis) by endoplasmic reticulum associated Δ6-desaturase activity. Currently sources of GLA are limited to a small number of plant species with poor agronomic properties, and therefore an economical and abundant commercial source of GLA in an existing crop is highly desirable. To this end, the seed oil of a high LA cultivated species of safflower (Carthamus tinctorius) was modified by transformation with Δ6-desaturase from Saprolegnia diclina resulting in levels exceeding 70% (v/v) of GLA. Levels around 50% (v/v) of GLA in seed oil was achieved when Δ12-/Δ6-desaturases from Mortierella alpina was over-expressed in safflower cultivars with either a high LA or high oleic (OA; C18:1Δ9 cis) background. The differences in the overall levels of GLA suggest the accumulation of the novel fatty acid was not limited by a lack of incorporation into the triacylgylcerol backbone (>66% GLA achieved), or correlated with gene dosage (GLA levels independent of gene copy number), but rather reflected the differences in Δ6-desaturase activity from the two sources. To date, these represent the highest accumulation levels of a newly introduced fatty acid in a transgenic crop. Events from these studies have been propagated and recently received FDA approval for commercialization as Sonova™400. |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Unknown | 41 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 24% |
| Student > Ph. D. Student | 7 | 17% |
| Researcher | 6 | 14% |
| Professor | 3 | 7% |
| Student > Doctoral Student | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 9 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 48% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Medicine and Dentistry | 2 | 5% |
| Environmental Science | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 11 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2020.
All research outputs
#7,452,489
of 22,785,242 outputs
Outputs from Transgenic Research
#366
of 891 outputs
Outputs of similar age
#43,145
of 124,041 outputs
Outputs of similar age from Transgenic Research
#8
of 11 outputs
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