| Title |
Cinnamic acid 4-hydroxylase from cell cultures of the hornwort Anthoceros agrestis
|
|---|---|
| Published in |
Planta, January 2003
|
| DOI | 10.1007/s00425-002-0960-9 |
| Pubmed ID | |
| Authors |
Maike Petersen |
| Abstract |
Cinnamic acid 4-hydroxylase (EC 1.14.13.11), a cytochrome P450-dependent hydroxylase was for the first time characterized from a hornwort, Anthoceros agrestis Paton (Anthocerotaceae). In suspension cultures of A. agrestis up to 5% of the dry weight was accumulated as rosmarinic acid, a natural product commonly known from higher plants (e.g. species of the Lamiaceae and Boraginaceae). Cinnamic acid 4-hydroxylase is involved in the biosynthesis of rosmarinic acid. The participation of cytochrome P450 was demonstrated by the inhibition of hydroxylase activity by cytochrome c and the inhibition of cinnamic acid hydroxylation in a CO-containing atmosphere, which is partially released by illumination with blue light. The apparent K(m) values were determined to be at 60 microM and 5 microM for NADPH and cinnamic acid, respectively. A comparatively high hydroxylation activity was seen with NADH as electron donor. While the hydroxylase activity with NADPH was strongly inhibited by the competitive electron acceptor cytochrome c, the activity with NADH was less susceptible, indicating the possibility of different electron-transfer pathways. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 6% |
| Norway | 1 | 6% |
| Unknown | 16 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 33% |
| Researcher | 4 | 22% |
| Professor > Associate Professor | 2 | 11% |
| Lecturer | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 61% |
| Chemistry | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 3 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2021.
All research outputs
#7,453,126
of 22,785,242 outputs
Outputs from Planta
#599
of 2,718 outputs
Outputs of similar age
#31,471
of 128,437 outputs
Outputs of similar age from Planta
#5
of 12 outputs
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So far Altmetric has tracked 2,718 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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