↓ Skip to main content

Regulation of Lateral Hypothalamic Orexin Activity by Local GABAergic Neurons

Overview of attention for article published in Journal of Neuroscience, January 2018
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)

Mentioned by

twitter
3 X users

Citations

dimensions_citation
43 Dimensions

Readers on

mendeley
76 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Regulation of Lateral Hypothalamic Orexin Activity by Local GABAergic Neurons
Published in
Journal of Neuroscience, January 2018
DOI 10.1523/jneurosci.1925-17.2017
Pubmed ID
Authors

Loris L. Ferrari, Daniel Park, Lin Zhu, Matthew R. Palmer, Rebecca Y. Broadhurst, Elda Arrigoni

Abstract

Orexin (also known as hypocretin) neurons are considered a key component of the ascending arousal system. They are active during wakefulness, at which time they drive and maintain arousal, and are silent during sleep. Their activity is controlled by long-range inputs from many sources, as well as by more short-range inputs, including from presumptive GABAergic neurons in the lateral hypothalamus/perifornical region (LH/PF). To characterize local GABAergic input to orexin neurons we employed channelrhodopsin-2 (ChR2)-assisted-circuit-mapping in brain slices. We expressed ChR2 in GABAergic neurons (Vgat+) in the LH/PF and recorded from genetically-identified surrounding orexin neurons (LH/PFVgat → Orx). We performed all experiments in mice of either sex. Photostimulation of LH/PF GABAergic neurons inhibited the firing of orexin neurons through the release of GABA, evoking GABAA-mediated inhibitory postsynaptic currents (IPSCs) in orexin neurons. These photo-evoked IPSCs were maintained in the presence of tetrodotoxin, indicating direct connectivity. Carbachol inhibited LH/PFVgat → Orx input through muscarinic receptors. By contrast, application of orexin was without effect on LH/PFVgat → Orx input, whereas dynorphin, another peptide produced by orexin neurons, inhibited LH/PFVgat → Orx input through κ-opioid receptors. Our results demonstrate that orexin neurons are under inhibitory control by local GABAergic neurons and that this input is depressed by cholinergic signaling, unaffected by orexin and inhibited by dynorphin. We propose that local release of dynorphin may, via collaterals, provide a positive feedback to orexin neurons and that during wakefulness orexin neurons may be disinhibited by acetylcholine and by their own release of dynorphin.SIGNIFICANCE STATEMENTThe lateral hypothalamus contains important wake-promoting cell populations, including orexin-producing neurons. Iintermingled with the orexin neurons there are other cell populations that selectively discharge during non-REM or REM sleep. Some of these sleep-active neurons release GABA and are thought to inhibit wake-active neurons during REM and non-REM sleep. However, this hypothesis had not been tested. Here we show that orexin neurons are inhibited by a local GABAergic input. We propose that this local GABAergic input inhibits orexin neurons during sleep, but that during wakefulness this input is depressed, possibly through cholinergically-mediated disinhibition and/or by release of dynorphin from orexin neurons themselves.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 76 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 24%
Student > Master 11 14%
Researcher 10 13%
Student > Doctoral Student 6 8%
Student > Bachelor 4 5%
Other 13 17%
Unknown 14 18%
Readers by discipline Count As %
Neuroscience 29 38%
Agricultural and Biological Sciences 13 17%
Medicine and Dentistry 6 8%
Biochemistry, Genetics and Molecular Biology 3 4%
Psychology 2 3%
Other 6 8%
Unknown 17 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2019.
All research outputs
#13,374,110
of 23,577,654 outputs
Outputs from Journal of Neuroscience
#16,504
of 23,491 outputs
Outputs of similar age
#210,985
of 445,061 outputs
Outputs of similar age from Journal of Neuroscience
#162
of 227 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 23,491 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.4. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 445,061 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 227 others from the same source and published within six weeks on either side of this one. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.