Title |
Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum
|
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Published in |
Naunyn-Schmiedeberg's Archives of Pharmacology, May 1992
|
DOI | 10.1007/bf00168954 |
Pubmed ID | |
Authors |
E. Müller-Schweinitzer, P. Ellis |
Abstract |
The cryoprotective effect of sucrose has been investigated using 3 different vascular smooth muscle preparations, namely canine saphenous veins and arteries and porcine circumflex coronary arteries following storage in liquid nitrogen (at -196 degrees C). Contractile responses to noradrenaline, 5-HT, prostaglandin F2 alpha and KCl and relaxant responses to substance P and 5-HT were determined on fresh tissues and after cryostorage in fetal calf serum (FCS) containing either 1.8 M dimethyl sulfoxide (DMSO), or 0.1 M sucrose or both agents combined. The data demonstrate that the addition of sucrose to the DMSO-containing cryomedium promotes the preservation of both contractile and relaxant activity of cryostored blood vessels, though sucrose alone did not confer any noticeable protection. |
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