| Title |
Metabolic engineering for improved fermentation of pentoses by yeasts
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, November 2003
|
| DOI | 10.1007/s00253-003-1450-0 |
| Pubmed ID | |
| Authors |
T. W. Jeffries, Y.-S. Jin |
| Abstract |
The fermentation of xylose is essential for the bioconversion of lignocellulose to fuels and chemicals, but wild-type strains of Saccharomyces cerevisiae do not metabolize xylose, so researchers have engineered xylose metabolism in this yeast. Glucose transporters mediate xylose uptake, but no transporter specific for xylose has yet been identified. Over-expressing genes for aldose (xylose) reductase, xylitol dehydrogenase and moderate levels of xylulokinase enable xylose assimilation and fermentation, but a balanced supply of NAD(P) and NAD(P)H must be maintained to avoid xylitol production. Reducing production of NADPH by blocking the oxidative pentose phosphate cycle can reduce xylitol formation, but this occurs at the expense of xylose assimilation. Respiration is critical for growth on xylose by both native xylose-fermenting yeasts and recombinant S, cerevisiae. Anaerobic growth by recombinant mutants has been reported. Reducing the respiration capacity of xylose-metabolizing yeasts increases ethanol production. Recently, two routes for arabinose metabolism have been engineered in S. cerevisiae and adapted strains of Pichia stipitis have been shown to ferment hydrolysates with ethanol yields of 0.45 g g(-1) sugar consumed, so commercialization seems feasible for some applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 398 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 1% |
| Mexico | 4 | 1% |
| Brazil | 3 | <1% |
| Chile | 2 | <1% |
| Canada | 2 | <1% |
| France | 2 | <1% |
| Italy | 1 | <1% |
| Latvia | 1 | <1% |
| South Africa | 1 | <1% |
| Other | 6 | 2% |
| Unknown | 371 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 101 | 25% |
| Researcher | 80 | 20% |
| Student > Master | 59 | 15% |
| Student > Bachelor | 43 | 11% |
| Student > Doctoral Student | 22 | 6% |
| Other | 58 | 15% |
| Unknown | 35 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 191 | 48% |
| Biochemistry, Genetics and Molecular Biology | 55 | 14% |
| Engineering | 31 | 8% |
| Chemistry | 27 | 7% |
| Chemical Engineering | 19 | 5% |
| Other | 27 | 7% |
| Unknown | 48 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2023.
All research outputs
#2,863,998
of 25,374,917 outputs
Outputs from Applied Microbiology and Biotechnology
#284
of 8,290 outputs
Outputs of similar age
#3,975
of 57,109 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#1
of 39 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,290 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 57,109 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.