Title |
Presence of a pre‐apoptotic complex of pro‐caspase‐3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells
|
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Published in |
EMBO Journal, April 1999
|
DOI | 10.1093/emboj/18.8.2040 |
Pubmed ID | |
Authors |
Afshin Samali, Jiyang Cai, Boris Zhivotovsky, Dean P. Jones, Sten Orrenius |
Abstract |
Activation of pro-caspase-3 is a central event in the execution phase of apoptosis and appears to serve as the convergence point of different apoptotic signaling pathways. Recently, mitochondria were found to play a central role in apoptosis through release of cytochrome c and activation of caspases. Moreover, a sub-population of pro-caspase-3 has been found to be localized to this organelle. In the present study, we demonstrate that pro-caspase-3 is present in the mitochondrial fraction of Jurkat T cells in a complex with the chaperone proteins Hsp60 and Hsp10. Induction of apoptosis with staurosporine led to the activation of mitochondrial pro-caspase-3 and its dissociation from the Hsps which were released from mitochondria. The release of Hsps occurred simultaneously with the release of other mitochondrial intermembrane space proteins including cytochrome c and adenylate kinase, prior to a loss of mitochondrial transmembrane potential. In in vitro systems, recombinant Hsp60 and Hsp10 accelerated the activation of pro-caspase-3 by cytochrome c and dATP in an ATP-dependent manner, consistent with their function as chaperones. This finding suggests that the release of mitochondrial Hsps may also accelerate caspase activation in the cytoplasm of intact cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 1% |
Germany | 2 | 1% |
South Africa | 2 | 1% |
Austria | 1 | <1% |
Unknown | 139 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 29 | 20% |
Researcher | 28 | 19% |
Student > Master | 26 | 18% |
Student > Bachelor | 17 | 12% |
Professor | 9 | 6% |
Other | 19 | 13% |
Unknown | 18 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 57 | 39% |
Biochemistry, Genetics and Molecular Biology | 33 | 23% |
Medicine and Dentistry | 11 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 6% |
Chemistry | 6 | 4% |
Other | 8 | 5% |
Unknown | 22 | 15% |