Title |
Genetic Program of Neuronal Differentiation and Growth Induced by Specific Activation of NMDA Receptors
|
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Published in |
Neurochemical Research, December 2006
|
DOI | 10.1007/s11064-006-9213-9 |
Pubmed ID | |
Authors |
Cristina A. Ghiani, Luis Beltran-Parrazal, Daniel M. Sforza, Jemily S. Malvar, Akop Seksenyan, Ruth Cole, Desmond J. Smith, Andrew Charles, Pedro A. Ferchmin, Jean de Vellis |
Abstract |
Glutamate and its receptors are expressed very early during development and may play important roles in neurogenesis, synapse formation and brain wiring. The levels of glutamate and activity of its receptors can be influenced by exogenous factors, leading to neurodevelopmental disorders. To investigate the role of NMDA receptors on gene regulation in a neuronal model, we used primary neuronal cultures developed from embryonic rat cerebri in serum-free medium. Using Affymetrix Gene Arrays, we found that genes known to be involved in neuronal plasticity were differentially expressed 24 h after a brief activation of NMDA receptors. The upregulation of these genes was accompanied by a sustained induction of CREB phosphorylation, and an increase in synaptophysin immunoreactivity. We conclude that NMDA receptor activation elicits expression of genes whose downstream products are involved in the regulation of early phases of the process leading to synaptogenesis and its consolidation, at least in part through sustained CREB phosphorylation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 3% |
Unknown | 35 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 19% |
Student > Master | 6 | 17% |
Student > Bachelor | 4 | 11% |
Professor | 4 | 11% |
Professor > Associate Professor | 3 | 8% |
Other | 7 | 19% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 12 | 33% |
Neuroscience | 5 | 14% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
Medicine and Dentistry | 2 | 6% |
Other | 4 | 11% |
Unknown | 6 | 17% |