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Mendeley readers
Attention Score in Context
| Title |
Molecular Characterization of TMPRSS2-ERG Gene Fusion in the NCI-H660 Prostate Cancer Cell Line: A New Perspective for an Old Model
|
|---|---|
| Published in |
Neoplasia, March 2007
|
| DOI | 10.1593/neo.07103 |
| Pubmed ID | |
| Authors |
Kirsten D. Mertz, Sunita R. Setlur, Saravana M. Dhanasekaran, Francesca Demichelis, Sven Perner, Scott Tomlins, Joëlle Tchinda, Bharathi Laxman, Robert L. Vessella, Rameen Beroukhimt, Charles Lee, Arul M. Chinnaiyan, Mark A. Rubin |
| Abstract |
Recent studies have established that a significant fraction of prostate cancers harbor a signature gene fusion between the 5' region of androgen-regulated TMPRSS2 and an ETS family transcription factor, most commonly ERG. Studies on the molecular mechanisms and functional consequences of this important chromosomal rearrangement are currently limited to the VCaP cell line derived from a vertebral bone metastasis of a hormone-refractory prostate tumor. Here we report on the NCI-H660 cell line, derived from a metastatic site of an extrapulmonary small cell carcinoma arising from the prostate. NCI-H660 harbors TMPRSS2-ERG fusion with a homozygous intronic deletion between TMPRSS2 and ERG. We demonstrate this by real-time quantitative polymerase chain reaction, a two-stage dual-color interphase fluorescence in situ hybridization (FISH) assay testing for TMPRSS2 and ERG break-aparts, and single-nucleotide polymorphism oligonucleotide arrays. The deletion is consistent with the common intronic deletion found on chromosome 21q22.2-3 in human prostate cancer samples. We demonstrate the physical juxtaposition of TMPRSS2 and ERG on the DNA level by fiber FISH. The androgen receptor-negative NCI-H660 cell line expresses ERG in an androgen-independent fashion. This in vitro model system has the potential to provide important pathobiologic insights into TMPRSS2-ERG fusion prostate cancer. |
Mendeley readers
The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| United Kingdom | 2 | 2% |
| Chile | 1 | <1% |
| Brazil | 1 | <1% |
| Canada | 1 | <1% |
| Portugal | 1 | <1% |
| Spain | 1 | <1% |
| China | 1 | <1% |
| Unknown | 114 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 34 | 27% |
| Student > Ph. D. Student | 27 | 22% |
| Student > Bachelor | 11 | 9% |
| Other | 10 | 8% |
| Professor > Associate Professor | 9 | 7% |
| Other | 21 | 17% |
| Unknown | 13 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 51 | 41% |
| Biochemistry, Genetics and Molecular Biology | 30 | 24% |
| Medicine and Dentistry | 17 | 14% |
| Computer Science | 3 | 2% |
| Immunology and Microbiology | 3 | 2% |
| Other | 3 | 2% |
| Unknown | 18 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 January 2012.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Neoplasia
#477
of 1,368 outputs
Outputs of similar age
#32,444
of 90,358 outputs
Outputs of similar age from Neoplasia
#9
of 15 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,368 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 90,358 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.