| Title |
Current achievements in the production of complex biopharmaceuticals with moss bioreactors
|
|---|---|
| Published in |
Bioprocess and Biosystems Engineering, August 2007
|
| DOI | 10.1007/s00449-007-0151-y |
| Pubmed ID | |
| Authors |
Eva L. Decker, Ralf Reski |
| Abstract |
Transgenic plants are promising alternatives for the low-cost and safe pathogen-free production of complex recombinant pharmaceutical proteins (molecular farming). Plants as higher eukaryotes perform posttranslational modifications similar to those of mammalian cells. However, plant-specific protein N-glycosylation was shown to be immunogenic, a fact that represents a drawback for many plant systems in biopharmaceutical production. The moss Physcomitrella patens offers unique properties as a contained system for protein production. It is grown in the predominant haploid gametophytic stage as tissue suspension cultures in photobioreactors. Efficient secretory signals and a transient transfection system allow the secretion of freshly synthesized proteins to the surrounding medium. The key advantage of Physcomitrella compared to other plant systems is the feasibility of targeted gene replacements. By this means, moss strains with non-immunogenic humanized glycan patterns were created. Here we present an overview of the relevant aspects for establishing moss as a production system for recombinant biopharmaceuticals. |
Mendeley readers
The data shown below were compiled from readership statistics for 152 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| Italy | 1 | <1% |
| Uruguay | 1 | <1% |
| Brazil | 1 | <1% |
| South Africa | 1 | <1% |
| Finland | 1 | <1% |
| United Kingdom | 1 | <1% |
| Denmark | 1 | <1% |
| Unknown | 143 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 31 | 20% |
| Student > Bachelor | 29 | 19% |
| Student > Ph. D. Student | 22 | 14% |
| Student > Master | 17 | 11% |
| Professor | 7 | 5% |
| Other | 23 | 15% |
| Unknown | 23 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 55 | 36% |
| Biochemistry, Genetics and Molecular Biology | 32 | 21% |
| Engineering | 13 | 9% |
| Chemical Engineering | 6 | 4% |
| Chemistry | 4 | 3% |
| Other | 11 | 7% |
| Unknown | 31 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2022.
All research outputs
#2,876,273
of 25,457,297 outputs
Outputs from Bioprocess and Biosystems Engineering
#3
of 8 outputs
Outputs of similar age
#6,502
of 79,134 outputs
Outputs of similar age from Bioprocess and Biosystems Engineering
#1
of 1 outputs
Altmetric has tracked 25,457,297 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8 research outputs from this source. They receive a mean Attention Score of 2.2. This one scored the same or higher as 5 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 79,134 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them