| Title |
Analysis of flavonoids in honey by HPLC coupled with coulometric electrode array detection and electrospray ionization mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, January 2011
|
| DOI | 10.1007/s00216-010-4614-7 |
| Pubmed ID | |
| Authors |
Karoline Petrus, Heidi Schwartz, Gerhard Sontag |
| Abstract |
The analysis of flavonoids in unifloral honeys by high-performance liquid chromatography (HPLC) coupled with coulometric electrode array detection (CEAD) is described. The compounds were extracted by a nonionic polymeric resin (Amberlite XAD-2) and then separated on a reversed phase column using gradient elution. Quercetin, naringenin, hesperetin, luteolin, kaempferol, isorhamnetin, and galangin were detected in a coulometric electrode array detection system between +300 and +800 mV against palladium reference electrodes, and their presence was additionally confirmed by HPLC coupled with electrospray ionization mass spectrometry. The method was applied to analysis of 19 honeys of different varieties and origin. The limits of detection and quantitation ranged between 1.6 and 8.3 μg/kg and 3.9 and 27.4 μg/kg, respectively. The recoveries were above 96% in fluid and above 89% in creamy honeys. Some of these honeys (melon, pumpkin, cherry blossom, dandelion, maple, and pine tree honey) were investigated for their flavonoid content and profile for the first time. Differences between honeys were observed both in flavonoid concentrations and in the flavonoid profiles. The flavonoid concentrations ranged from 0.015 to 3.4 mg/kg honey. Galangin, kaempferol, quercetin, isorhamnetin, and luteolin were detected in all investigated honeys, whereas hesperetin occurred only in lemon and orange honeys and naringenin in lemon, orange, rhododendron, rosemary, and cherry blossom honeys. |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Portugal | 1 | 1% |
| China | 1 | 1% |
| Unknown | 88 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 18% |
| Student > Master | 15 | 16% |
| Student > Bachelor | 9 | 10% |
| Student > Doctoral Student | 8 | 9% |
| Student > Postgraduate | 7 | 8% |
| Other | 15 | 16% |
| Unknown | 21 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 20 | 22% |
| Agricultural and Biological Sciences | 13 | 14% |
| Biochemistry, Genetics and Molecular Biology | 8 | 9% |
| Medicine and Dentistry | 8 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Other | 14 | 15% |
| Unknown | 25 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2020.
All research outputs
#8,535,684
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#2,202
of 9,619 outputs
Outputs of similar age
#58,647
of 192,310 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#24
of 55 outputs
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