Title |
Synthetic biology of polyketide synthases
|
---|---|
Published in |
Journal of Industrial Microbiology & Biotechnology, July 2018
|
DOI | 10.1007/s10295-018-2021-9 |
Pubmed ID | |
Authors |
Satoshi Yuzawa, Tyler W H Backman, Jay D Keasling, Leonard Katz |
Abstract |
Complex reduced polyketides represent the largest class of natural products that have applications in medicine, agriculture, and animal health. This structurally diverse class of compounds shares a common methodology of biosynthesis employing modular enzyme systems called polyketide synthases (PKSs). The modules are composed of enzymatic domains that share sequence and functional similarity across all known PKSs. We have used the nomenclature of synthetic biology to classify the enzymatic domains and modules as parts and devices, respectively, and have generated detailed lists of both. In addition, we describe the chassis (hosts) that are used to assemble, express, and engineer the parts and devices to produce polyketides. We describe a recently developed software tool to design PKS system and provide an example of its use. Finally, we provide perspectives of what needs to be accomplished to fully realize the potential that synthetic biology approaches bring to this class of molecules. |
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Geographical breakdown
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United States | 6 | 23% |
Germany | 3 | 12% |
United Kingdom | 2 | 8% |
Brazil | 1 | 4% |
Korea, Republic of | 1 | 4% |
Australia | 1 | 4% |
Unknown | 12 | 46% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 17 | 65% |
Scientists | 9 | 35% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 103 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 28 | 27% |
Student > Master | 19 | 18% |
Researcher | 13 | 13% |
Student > Bachelor | 11 | 11% |
Student > Doctoral Student | 5 | 5% |
Other | 8 | 8% |
Unknown | 19 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 31 | 30% |
Chemistry | 17 | 17% |
Agricultural and Biological Sciences | 15 | 15% |
Unspecified | 4 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
Other | 12 | 12% |
Unknown | 21 | 20% |