| Title |
Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, September 1995
|
| DOI | 10.1007/bf00164771 |
| Pubmed ID | |
| Authors |
F. G. Healy, R. M. Ray, H. C. Aldrich, A. C. Wilkie, L. O. Ingram, K. T. Shanmugam |
| Abstract |
Gene libraries ("zoolibraries") were constructed in Escherichia coli using DNA isolated from the mixed liquor of thermophilic, anaerobic digesters, which were in continuous operation with lignocellulosic feedstocks for over 10 years. Clones expressing cellulase and xylosidase were readily recovered from these libraries. Four clones that hydrolyzed carboxymethylcellulose and methylumbelliferyl-beta-D-cellobiopyranoside were characterized. All four cellulases exhibited temperature optima (60-65 degrees C) and pH optima (pH 6-7) in accordance with conditions of the enrichment. The DNA sequence of the insert in one clone (plasmid pFGH1) was determined. This plasmid encoded an endoglucanase (celA) and part of a putative beta-glucosidase (celB), both of which were distinctly different from all previously reported homologues. CelA protein shared limited homology with members of the A3 subfamily of cellulases, being similar to endoglucanase C from Clostridium thermocellum (40% identity). The N-terminal part of CelB protein was most similar to beta-glucosidase from Pseudomonas fluorescens subsp. cellulosa (28% homology). The use of zoolibraries constructed from natural or laboratory enrichment cultures offers the potential to discover many new enzymes for biotechnological applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 166 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 4% |
| Spain | 2 | 1% |
| Germany | 1 | <1% |
| France | 1 | <1% |
| Hong Kong | 1 | <1% |
| Brazil | 1 | <1% |
| Switzerland | 1 | <1% |
| Mexico | 1 | <1% |
| Malaysia | 1 | <1% |
| Other | 2 | 1% |
| Unknown | 149 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 39 | 23% |
| Researcher | 29 | 17% |
| Student > Master | 26 | 16% |
| Student > Bachelor | 15 | 9% |
| Student > Doctoral Student | 10 | 6% |
| Other | 26 | 16% |
| Unknown | 21 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 71 | 43% |
| Biochemistry, Genetics and Molecular Biology | 39 | 23% |
| Immunology and Microbiology | 8 | 5% |
| Environmental Science | 7 | 4% |
| Computer Science | 4 | 2% |
| Other | 12 | 7% |
| Unknown | 25 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2020.
All research outputs
#8,022,830
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,748
of 8,034 outputs
Outputs of similar age
#7,376
of 24,546 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#4
of 23 outputs
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So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.