| Title |
RNA Polymerase of Aquifex pyrophilus: Implications for the Evolution of the Bacterial rpoBC Operon and Extremely Thermophilic Bacteria
|
|---|---|
| Published in |
Journal of Molecular Evolution, May 1999
|
| DOI | 10.1007/pl00006496 |
| Pubmed ID | |
| Authors |
Hans-Peter Klenk, Thomas-Dirk Meier, Peter Durovic, Volker Schwass, Friedrich Lottspeich, Patrick P. Dennis, Wolfram Zillig |
| Abstract |
A 16,226-bp fragment from the genome of Aquifex pyrophilus was sequenced, containing the genes for ribosomal proteins L1, L10, and L7/12 (rplAJL), DNA-directed RNA polymerase subunits beta and beta' (rpoBC), alanyl-tRNA synthetase (alaS), and subunit A of proteinase Clp (clpA). Enzymatic activity and extreme thermostability of purified A. pyrophilus RNA polymerase were verified. Transcription initiation on a DNA construct harboring the T7 A1 promoter was demonstrated by elongation of a 32P-labeled trinucleotide. Phylogenetic analyses of the two largest subunits of bacterial RNA polymerases (beta and beta') showed overall consistency with the 16S rRNA-based phylogeny, except for the positions of the hyperthermophiles A. pyrophilus and Thermotoga maritima and for the location of the root of the domain Bacteria. In the phylogenies for both RNA polymerase subunits beta and beta', A. pyrophilus was placed within the Gram-negative bacteria below the epsilon subdivision of the Proteobacteria. No support was found for the 16S rRNA-based hypothesis that A. pyrophilus might be the deepest branch of the Bacteria, but the cell wall-less mycoplasmas were found with a high confidence at the root of the Bacteria phylogenies. This raised doubts not only about whether the original Bacteria were indeed like the hyperthermophiles, but also concerning the value of single-gene phylogenies for hypotheses about the evolution of organisms. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Finland | 1 | 3% |
| Mexico | 1 | 3% |
| United States | 1 | 3% |
| Netherlands | 1 | 3% |
| Unknown | 32 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 33% |
| Professor | 10 | 28% |
| Student > Master | 6 | 17% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Doctoral Student | 1 | 3% |
| Other | 3 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 61% |
| Biochemistry, Genetics and Molecular Biology | 7 | 19% |
| Earth and Planetary Sciences | 3 | 8% |
| Environmental Science | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 1 | 3% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2013.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Journal of Molecular Evolution
#493
of 1,477 outputs
Outputs of similar age
#11,895
of 36,587 outputs
Outputs of similar age from Journal of Molecular Evolution
#9
of 18 outputs
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