| Title |
The Remarkable Transport Mechanism of P-Glycoprotein: A Multidrug Transporter
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, December 2005
|
| DOI | 10.1007/s10863-005-9497-5 |
| Pubmed ID | |
| Authors |
Marwan K. Al-Shawi, Hiroshi Omote |
| Abstract |
Human P-glycoprotein (ABCB1) is a primary multidrug transporter located in plasma membranes, that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. P-glycoprotein employs a most unusual molecular mechanism to perform this drug transport function. Here we review our work to elucidate the molecular mechanism of drug transport by P-glycoprotein. High level heterologous expression of human P-glycoprotein, in the yeast Saccharomyces cerevisiae, has facilitated biophysical studies in purified proteoliposome preparations. Development of novel spin-labeled transport substrates has allowed for quantitative and rigorous measurements of drug transport in real time by EPR spectroscopy. We have developed a new drug transport model of P-glycoprotein from the results of mutagenic, quantitative thermodynamic and kinetic studies. This model satisfactorily accounts for most of the unusual kinetic, coupling, and physiological features of P-glycoprotein. Additionally, an atomic detail structural model of P-glycoprotein has been devised to place our results within a proper structural context. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 4% |
| Germany | 1 | 2% |
| Portugal | 1 | 2% |
| United Kingdom | 1 | 2% |
| Poland | 1 | 2% |
| Unknown | 51 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 13 | 23% |
| Student > Ph. D. Student | 11 | 19% |
| Researcher | 7 | 12% |
| Student > Master | 7 | 12% |
| Student > Doctoral Student | 5 | 9% |
| Other | 11 | 19% |
| Unknown | 3 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 30% |
| Biochemistry, Genetics and Molecular Biology | 14 | 25% |
| Chemistry | 9 | 16% |
| Medicine and Dentistry | 5 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 7 | 12% |
| Unknown | 3 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2014.
All research outputs
#7,926,100
of 23,867,274 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#105
of 466 outputs
Outputs of similar age
#39,384
of 150,868 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#2
of 6 outputs
Altmetric has tracked 23,867,274 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 466 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.