| Title |
Characterization of the human ADAMTS-5 (aggrecanase-2) gene promoter
|
|---|---|
| Published in |
Molecular Biology Reports, January 2007
|
| DOI | 10.1007/s11033-006-9037-3 |
| Pubmed ID | |
| Authors |
Kannan Thirunavukkarasu, Yong Pei, Tao Wei |
| Abstract |
Aggrecan degradation in osteoarthritis is mediated primarily by aggrecanases (-1 and -2) that are members of the ADAMTS family of proteases. Aggrecanase-2 (ADAMTS-5) null mice are resistant to aggrecan degradation in models of experimentally-induced osteoarthritis. In order to analyze ADAMTS-5 gene expression at the transcriptional level, we have cloned a 2.6 kb promoter region (-2096 to +506) of the human ADAMTS-5 gene and generated betagal reporter constructs. Promoter functionality was tested in transient transfection assays in chondrocytic cells. Analysis of the 2.6 kb promoter sequence indicated four putative binding sites for the Runx family of transcription factors, of which one member, Runx2, plays a role in chondrocyte maturation and hypertrophy. Overexpression of Runx2 stimulated reporter gene expression approximately 7-fold over control in SW1353 human chondrosarcoma cells and approximately 5-fold over control in primary bovine articular chondrocytes, suggesting ADAMTS-5 to be a potential downstream target of Runx2. 5'-deletions in the promoter resulted in a substantial loss in responsiveness to Runx2 implicating the functional importance of the distal promoter region that harbors putative Runx binding elements for achieving maximal Runx2 effects. The promoter-reporter construct could serve as a useful tool to understand the regulation of ADAMTS-5 gene expression at the transcriptional level. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 19% |
| Researcher | 4 | 13% |
| Other | 3 | 10% |
| Student > Doctoral Student | 3 | 10% |
| Professor > Associate Professor | 3 | 10% |
| Other | 5 | 16% |
| Unknown | 7 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 26% |
| Agricultural and Biological Sciences | 8 | 26% |
| Medicine and Dentistry | 6 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Arts and Humanities | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 5 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2015.
All research outputs
#6,413,206
of 22,785,242 outputs
Outputs from Molecular Biology Reports
#316
of 2,905 outputs
Outputs of similar age
#35,472
of 158,057 outputs
Outputs of similar age from Molecular Biology Reports
#2
of 6 outputs
Altmetric has tracked 22,785,242 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 2,905 research outputs from this source. They receive a mean Attention Score of 2.2. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 158,057 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.