| Title |
Characterization of Functional, Safety, and Probiotic Properties of Enterococcus faecalis AG5 Isolated From Wistar Rat, Demonstrating Adherence to HCT 116 Cells and Gastrointestinal Survivability
|
|---|---|
| Published in |
Probiotics and Antimicrobial Proteins, February 2018
|
| DOI | 10.1007/s12602-018-9387-x |
| Pubmed ID | |
| Authors |
Alok Kumar Mishra, Asit Ranjan Ghosh |
| Abstract |
Gut microbiota remains a prominent source for a diverse range of potential probiotics. In this context, the current study explored the rectal region of experimental Wistar rat for the isolation of potent probiotic. Sixteen lactic acid bacteria (LAB), from rectal swab of Wistar rats, were subjected to evaluation of probiotic properties. Among all, AG5 was found unique with consistent probiotic properties and was further identified as Enterococcus faecalis AG5 (NCBI accession number KT248537) using 16S rDNA sequencing, followed by BLAST analysis. Since the Enterococci strains inhibit various food-borne pathogens efficiently while proving itself as a safe probiotic candidate, the study further evaluated the safety of the strain AG5 using primer specific PCR amplification which revealed the existence of gene encoding gelE, asa1, efaA, ace, vanA, and vanB and negative for cylA, hyl, and esp respectively. SEM analysis confirmed the adherence ability of AG5 to HCT 116 cells. Adherence was found to be non-colonial and scattered manner. Furthermore, the strain demonstrated a significant survivability during simulated gastrointestinal transit. Taken together, the E. faecalis AG5 was found potential probiotic candidate with future implication in both food and health industry. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 18% |
| Student > Master | 6 | 13% |
| Student > Bachelor | 4 | 9% |
| Other | 4 | 9% |
| Lecturer | 2 | 4% |
| Other | 7 | 16% |
| Unknown | 14 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 20% |
| Immunology and Microbiology | 6 | 13% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Medicine and Dentistry | 2 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 19 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2018.
All research outputs
#15,492,327
of 23,023,224 outputs
Outputs from Probiotics and Antimicrobial Proteins
#237
of 546 outputs
Outputs of similar age
#271,530
of 442,600 outputs
Outputs of similar age from Probiotics and Antimicrobial Proteins
#15
of 31 outputs
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So far Altmetric has tracked 546 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
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We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.