Title |
Characterization of bacterial composition and diversity in a long-term petroleum contaminated soil and isolation of high-efficiency alkane-degrading strains using an improved medium
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Published in |
World Journal of Microbiology and Biotechnology, February 2018
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DOI | 10.1007/s11274-018-2417-8 |
Pubmed ID | |
Authors |
Jun Zheng, Jun-Qiao Feng, Lei Zhou, Serge Maurice Mbadinga, Ji-Dong Gu, Bo-Zhong Mu |
Abstract |
Bacterial community and diversity in a long-term petroleum-contaminated soil of an oilfield were characterized using 16S rRNA gene-based Illumina MiSeq high-throughput sequencing. Results indicated that Proteobacteria (49.11%) and Actinobacteria (24.24%) were the most dominant phyla, and the most abundant genera were Pseudoxanthomonas (8.47%), Luteimonas (3.64%), Alkanindiges (9.76%), Acinetobacter (5.26%) and Agromyces (8.56%) in the soil. Meanwhile a series of cultivations were carried out for isolation of alkane degraders from petroleum-contaminated soil with gellan gum and agar as gelling agents. And the isolates were classified by their 16S rRNA genes. Nine of the isolates including Enterobacter, Pseudomonas,Acinetobacter, Rhizobium, Bacillus, Sphingomonas, Paenibacillus, Variovorax and Rhodococcus showed strong biodegradability of alkane mixture (C9-C30) in a wide range of chain-length, which could be potentially applied in enhancement of bioremediation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 64 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 33% |
Student > Master | 8 | 13% |
Researcher | 5 | 8% |
Student > Bachelor | 4 | 6% |
Lecturer | 3 | 5% |
Other | 7 | 11% |
Unknown | 16 | 25% |
Readers by discipline | Count | As % |
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Environmental Science | 13 | 20% |
Biochemistry, Genetics and Molecular Biology | 12 | 19% |
Agricultural and Biological Sciences | 10 | 16% |
Immunology and Microbiology | 3 | 5% |
Earth and Planetary Sciences | 3 | 5% |
Other | 4 | 6% |
Unknown | 19 | 30% |