| Title |
Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia
|
|---|---|
| Published in |
BMC Plant Biology, February 2018
|
| DOI | 10.1186/s12870-018-1235-3 |
| Pubmed ID | |
| Authors |
Yongqing Wan, Mingzhu Mao, Dongli Wan, Qi Yang, Feiyun Yang, Mandlaa, Guojing Li, Ruigang Wang |
| Abstract |
WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species. We identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively. Fifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 19% |
| Student > Master | 4 | 11% |
| Student > Bachelor | 3 | 8% |
| Student > Doctoral Student | 3 | 8% |
| Professor > Associate Professor | 3 | 8% |
| Other | 6 | 17% |
| Unknown | 10 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 36% |
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Arts and Humanities | 1 | 3% |
| Unspecified | 1 | 3% |
| Environmental Science | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 13 | 36% |
Attention Score in Context
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#20,465,050
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Outputs from BMC Plant Biology
#2,548
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Outputs of similar age from BMC Plant Biology
#39
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