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Molecular cloning and domain structure of chicken pyruvate carboxylase

Overview of attention for article published in Biochemical & Biophysical Research Communications, July 2002
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Title
Molecular cloning and domain structure of chicken pyruvate carboxylase
Published in
Biochemical & Biophysical Research Communications, July 2002
DOI 10.1016/s0006-291x(02)00651-4
Pubmed ID
Authors

Sarawut Jitrapakdee, Mark G Nezic, A Ian Cassady, Yeesim Khew-Goodall, John C Wallace

Abstract

Pyruvate carboxylase (PC) [EC 6.4.1.1] is a biotin-dependent carboxylase that catalyses the conversion of pyruvate to oxaloacetate. Here we have determined the complete nucleotide sequence encoding chicken PC (cPC) by screening a liver cDNA library, by RT-PCR of poly(A)(+) RNA, and by PCR of genomic DNA. The full-length transcript contains an open reading frame of 3537 nucleotides, including the stop codon, encoding a polypeptide of 1178 amino acids with M(r) of 127,262. The amino acid sequence of cPC shows approximately 77% identity to mammalian PC. Limited proteolysis of pure cPC with chymotrypsin yields a major stable 75 kDa C-terminal peptide, including the biotinyl domain and a minor, unstable 39 kDa N-terminal peptide. Northern analysis of poly(A)(+) RNA isolated from chicken liver has shown that cPC's mRNA is approximately 5 kb in length, including a very long 3'-untranslated region.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 27%
Student > Ph. D. Student 3 20%
Other 2 13%
Professor 1 7%
Student > Postgraduate 1 7%
Other 0 0%
Unknown 4 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 33%
Agricultural and Biological Sciences 3 20%
Chemistry 1 7%
Medicine and Dentistry 1 7%
Unknown 5 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2023.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Biochemical & Biophysical Research Communications
#7,621
of 26,637 outputs
Outputs of similar age
#16,877
of 47,904 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#44
of 146 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 26,637 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 47,904 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.