| Title |
Electron capture dissociation of gaseous multiply charged ions by Fourier-transform ion cyclotron resonance
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, March 2001
|
| DOI | 10.1016/s1044-0305(00)00223-3 |
| Pubmed ID | |
| Authors |
Fred W. McLafferty, David M. Horn, Kathrin Breuker, Ying Ge, Mark A. Lewis, Blas Cerda, Roman A. Zubarev, Barry K. Carpenter |
| Abstract |
Fourier-transform ion cyclotron resonance instrumentation is uniquely applicable to an unusual new ion chemistry, electron capture dissociation (ECD). This causes nonergodic dissociation of far larger molecules (42 kDa) than previously observed (<1 kDa), with the resulting unimolecular ion chemistry also unique because it involves radical site reactions for similarly larger ions. ECD is highly complementary to the well known energetic methods for multiply charged ion dissociation, providing much more extensive protein sequence information, including the direct identification of N- versus C-terminal fragment ions. Because ECD only excites the molecule near the cleavage site, accompanying rearrangements are minimized. Counterintuitively, cleavage of backbone covalent bonds of protein ions is favored over that of noncovalent bonds; larger (>10 kDa) ions give far more extensive ECD if they are first thermally activated. This high specificity for covalent bond cleavage also makes ECD promising for studying the secondary and tertiary structure of gaseous protein ions caused by noncovalent bonding. |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 3% |
| United Kingdom | 1 | <1% |
| France | 1 | <1% |
| Belgium | 1 | <1% |
| Unknown | 102 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 33 | 31% |
| Researcher | 21 | 19% |
| Student > Bachelor | 11 | 10% |
| Student > Master | 9 | 8% |
| Student > Doctoral Student | 6 | 6% |
| Other | 20 | 19% |
| Unknown | 8 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 55 | 51% |
| Agricultural and Biological Sciences | 17 | 16% |
| Biochemistry, Genetics and Molecular Biology | 16 | 15% |
| Computer Science | 2 | 2% |
| Medicine and Dentistry | 2 | 2% |
| Other | 5 | 5% |
| Unknown | 11 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2021.
All research outputs
#5,447,195
of 25,374,917 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#555
of 3,834 outputs
Outputs of similar age
#7,142
of 42,454 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#1
of 13 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,834 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 42,454 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.