Title |
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus
|
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Published in |
Archives of Virology, February 2018
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DOI | 10.1007/s00705-018-3761-6 |
Pubmed ID | |
Authors |
Steven Van Borm, Mieke Steensels, Elisabeth Mathijs, Claude Kwe Yinda, Jelle Matthijnssens, Bénédicte Lambrecht |
Abstract |
Using random high-throughput RNA sequencing, the complete coding sequence of a novel picorna-like virus (a 9,228-nt contig containing 212,202 reads) was determined from a blackbird (Turdus merula) infected with Usutu virus. This sequence shares only 36% amino acid sequence identity with its closest homolog, arivirus 1, (an unclassified member of the order Picornavirales), and shares its dicistronic genome arrangement. The new virus was therefore tentatively named "blackbird arilivirus" (ari-like virus). The nearly complete genome sequence consists of at least 9,228 nt and contains two open reading frames (ORFs) encoding the nonstructural polyprotein (2235 amino acids) and structural polyprotein (769 amino acids). Two TaqMan RT-qPCR assays specific for ORF1 confirmed the presence of high levels of this novel virus in the original sample. Nucleotide composition analysis suggests that blackbird arilivirus is of dietary (plant) origin. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 27% |
Student > Doctoral Student | 2 | 13% |
Student > Bachelor | 2 | 13% |
Student > Master | 2 | 13% |
Researcher | 2 | 13% |
Other | 1 | 7% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 27% |
Agricultural and Biological Sciences | 3 | 20% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Computer Science | 1 | 7% |
Engineering | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 33% |