| Title |
The Preparation of Acellular Dermal Matrices by Freeze–Thawing and Ultrasonication Process and the Evaluation of Its Antigenicity
|
|---|---|
| Published in |
Cell Biochemistry and Biophysics, February 2015
|
| DOI | 10.1007/s12013-015-0569-9 |
| Pubmed ID | |
| Authors |
Yazhen Xu, Guoan Zhang, Yang Chang, Yu-xuan Qiu, Cheng Wang |
| Abstract |
Antigenicity is the biggest obstacle of xenogeneic acellular dermal matrices (ADM) as dermal scaffold in treatment of large-area skin defect. We prepared ADM by repeated freezing and thawing and ultrasonic process, and then injected the ADM homogenate and degradation product into porcine skin to evaluate the effectiveness of the decellularized method and the antigenicity of porcine ADM. In this work, chinese miniature pigs (n = 10) were sensitized by subcutaneous injection with human ADM degradation products on days 0, 7, and 14. After 21 days, their abdominal skin was divided into five regions for intradermal injection of porcine ADM homogenate (PADM), PADM degradation products, human ADM homogenate (HADM), HADM degradation products, and physiological saline (negative control). Positive controls (n = 2) were processed with fresh human skin homogenate by the same method. The skin manifestations in related areas were observed at 24 and 48 h and then the skin was subjected to histopathological and immunohistochemical analysis. The results showed that skin erythema and hydroderma were not observed in all groups but in positive control group. The histopathological and immunohistochemical results confirmed that no inflammatory cell infiltration, irregular extracellular matrix, IL-2, and IFN-γ expression were observed in all four test groups. Our results suggest that the combination with repeated freeze-thawing and ultrasonication can be an effective method to prepare ADM, which has great potential in clinical application. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 21% |
| Student > Ph. D. Student | 1 | 7% |
| Unspecified | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 7 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 3 | 21% |
| Medicine and Dentistry | 3 | 21% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Unspecified | 1 | 7% |
| Chemistry | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 5 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2015.
All research outputs
#15,269,109
of 22,786,087 outputs
Outputs from Cell Biochemistry and Biophysics
#356
of 910 outputs
Outputs of similar age
#208,667
of 352,279 outputs
Outputs of similar age from Cell Biochemistry and Biophysics
#19
of 121 outputs
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So far Altmetric has tracked 910 research outputs from this source. They receive a mean Attention Score of 2.1. This one has gotten more attention than average, scoring higher than 59% of its peers.
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