| Title |
Cloning and Characterization of the Xyn11A Gene from Lentinula edodes
|
|---|---|
| Published in |
The Protein Journal, January 2005
|
| DOI | 10.1007/s10930-004-0602-0 |
| Pubmed ID | |
| Authors |
Charles C. Lee, Dominic W. S. Wong, George H. Robertson |
| Abstract |
Hemicellulose represents a rich source of biomass that can be converted into useful chemical feedstocks. One of the main components of hemicellulose is xylan, a polymer of xylose residues. Xylanase enzymes that hydrolyze xylan are therefore of great commercial interest. We have cloned a gene (xyn11A) that encodes a 283-amino acid xylanase enzyme from the fungus Lentinula edodes. The enzyme has a pI of 4.6 and belongs to the highly conserved glycosyl hydrolase family 11. The xylanase gene was cloned into a Pichia pastoris expression vector that secretes active enzyme into both solid and liquid media. The optimal reaction conditions were at pH 4.5 and 50 degrees C. The enzyme had a Km of 1.5 mg/ml and a Vmax of 2.1 mmol/min/mg. Xyn11A produced primarily xylobiose, xylotriose, and xylotetraose from a birchwood xylan substrate. This is the first report on the cloning of a hemicellulase gene from L. edodes. |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Finland | 1 | 2% |
| Canada | 1 | 2% |
| Russia | 1 | 2% |
| Japan | 1 | 2% |
| Unknown | 37 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 24% |
| Student > Ph. D. Student | 7 | 17% |
| Student > Bachelor | 5 | 12% |
| Student > Postgraduate | 5 | 12% |
| Student > Master | 5 | 12% |
| Other | 5 | 12% |
| Unknown | 5 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 50% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Chemistry | 4 | 10% |
| Arts and Humanities | 1 | 2% |
| Immunology and Microbiology | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 7 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2021.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from The Protein Journal
#163
of 639 outputs
Outputs of similar age
#38,859
of 151,229 outputs
Outputs of similar age from The Protein Journal
#2
of 3 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 639 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 151,229 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.