| Title |
Gigantol inhibits Wnt/β-catenin signaling and exhibits anticancer activity in breast cancer cells
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, February 2018
|
| DOI | 10.1186/s12906-018-2108-x |
| Pubmed ID | |
| Authors |
Shubin Yu, Zhongyuan Wang, Zijie Su, Jiaxing Song, Liang Zhou, Qi Sun, Shanshan Liu, Shiyue Li, Ying Li, Meina Wang, Guo-Qiang Zhang, Xue Zhang, Zhong-Jian Liu, Desheng Lu |
| Abstract |
Gigantol is a bibenzyl compound derived from several medicinal orchids. This biologically active compound has been shown to have promising therapeutic potential against cancer cells, but its mechanism of action remains unclear. The inhibitory effect of gigantol on Wnt/β-catenin signaling was evaluated with the SuperTOPFlash reporter system. The levels of phosphorylated low-density lipoprotein receptor related protein 6 (LRP6), total LRP6 and cytosolic β-catenin were determined by Western blot analysis. The expression of Wnt target genes was analyzed using real-time PCR. Cell viability was measured with a MTT assay. The effect of gigantol on cell migration was examined using scratch wound-healing and transwell migration assays. Gigantol decreased the level of phosphorylated LRP6 and cytosolic β-catenin in HEK293 cells. In breast cancer MDA-MB-231 and MDA-MB-468 cells, treatment with gigantol reduced the level of phosphorylated LRP6, total LRP6 and cytosolic β-catenin in a dose-dependent manner, resulting in a decrease in the expression of Wnt target genes Axin2 and Survivin. We further demonstrated that gigantol suppressed the viability and migratory capacity of breast cancer cells. Gigantol is a novel inhibitor of the Wnt/β-catenin pathway. It inhibits Wnt/β-catenin signaling through downregulation of phosphorylated LRP6 and cytosolic β-catenin in breast cancer cells. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 13% |
| Researcher | 4 | 13% |
| Student > Doctoral Student | 3 | 9% |
| Student > Ph. D. Student | 3 | 9% |
| Professor > Associate Professor | 2 | 6% |
| Other | 6 | 19% |
| Unknown | 10 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 16% |
| Medicine and Dentistry | 5 | 16% |
| Agricultural and Biological Sciences | 3 | 9% |
| Nursing and Health Professions | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 9 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#3,732,822
of 23,023,224 outputs
Outputs from BMC Complementary Medicine and Therapies
#717
of 3,644 outputs
Outputs of similar age
#86,270
of 446,257 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#25
of 109 outputs
Altmetric has tracked 23,023,224 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,644 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 446,257 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.