| Title |
Visualization of endothelial cell cycle dynamics in mouse using the Flt-1/eGFP-anillin system
|
|---|---|
| Published in |
Angiogenesis, February 2018
|
| DOI | 10.1007/s10456-018-9601-1 |
| Pubmed ID | |
| Authors |
Katia Herz, Alexandra Becker, Chenyue Shi, Masatsugo Ema, Satoru Takahashi, Michael Potente, Michael Hesse, Bernd K. Fleischmann, Daniela Wenzel |
| Abstract |
Endothelial cell proliferation is a key process during vascular growth but its kinetics could only be assessed in vitro or ex vivo so far. To enable the monitoring and quantification of cell cycle kinetics in vivo, we have generated transgenic mice expressing an eGFP-anillin construct under control of the endothelial-specific Flt-1 promoter. This construct labels the nuclei of endothelial cells in late G1, S and G2 phase and changes its localization during the different stages of M phase, thereby enabling the monitoring of EC proliferation and cytokinesis. In Flt-1/eGFP-anillin mice, we found eGFP+ signals specifically in Ki67+/PECAM+ endothelial cells during vascular development. Quantification using this cell cycle reporter in embryos revealed a decline in endothelial cell proliferation between E9.5 to E12.5. By time-lapse microscopy, we determined the length of different cell cycle phases in embryonic endothelial cells in vivo and found a M phase duration of about 80 min with 2/3 covering karyokinesis and 1/3 cytokinesis. Thus, we have generated a versatile transgenic system for the accurate assessment of endothelial cell cycle dynamics in vitro and in vivo. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 25% |
| Student > Master | 6 | 21% |
| Researcher | 3 | 11% |
| Student > Bachelor | 2 | 7% |
| Professor | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 18% |
| Environmental Science | 4 | 14% |
| Agricultural and Biological Sciences | 4 | 14% |
| Medicine and Dentistry | 2 | 7% |
| Engineering | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 7 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2018.
All research outputs
#20,465,050
of 23,023,224 outputs
Outputs from Angiogenesis
#442
of 538 outputs
Outputs of similar age
#375,893
of 437,841 outputs
Outputs of similar age from Angiogenesis
#16
of 18 outputs
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