| Title |
Regulation of mitochondrial aconitase by phosphorylation in diabetic rat heart
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, January 2009
|
| DOI | 10.1007/s00018-009-8696-3 |
| Pubmed ID | |
| Authors |
G. Lin, R. W. Brownsey, K. M. MacLeod |
| Abstract |
Mitochondrial dysfunction and protein kinase C (PKC) activation are consistently found in diabetic cardiomyopathy but their relationship remains unclear. This study identified mitochondrial aconitase as a downstream target of PKC activation using immunoblotting and mass spectrometry, and then characterized phosphorylation-induced changes in its activity in hearts from type 1 diabetic rats. PKCbeta(2) co-immunoprecipitated with phosphorylated aconitase from mitochondria isolated from diabetic hearts. Augmented phosphorylation of mitochondrial aconitase in diabetic hearts was found to be associated with an increase in its reverse activity (isocitrate to aconitate), while the rate of the forward activity was unchanged. Similar results were obtained on phosphorylation of mitochondrial aconitase by PKCbeta(2) in vitro. These results demonstrate the regulation of mitochondrial aconitase activity by PKC-dependent phosphorylation. This may influence the activity of the tricarboxylic acid cycle, and contribute to impaired mitochondrial function and energy metabolism in diabetic hearts. |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 3% |
| Spain | 1 | 3% |
| Unknown | 35 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 19% |
| Student > Bachelor | 5 | 14% |
| Student > Master | 5 | 14% |
| Professor > Associate Professor | 4 | 11% |
| Student > Ph. D. Student | 3 | 8% |
| Other | 6 | 16% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 32% |
| Agricultural and Biological Sciences | 8 | 22% |
| Medicine and Dentistry | 5 | 14% |
| Chemical Engineering | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 7 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2015.
All research outputs
#7,845,540
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#1,655
of 4,151 outputs
Outputs of similar age
#51,042
of 175,042 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#14
of 37 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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