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Mendeley readers
Attention Score in Context
| Title |
A New PCR-Based Approach Indicates the Range of Clonorchis sinensis Now Extends to Central Thailand
|
|---|---|
| Published in |
PLoS Neglected Tropical Diseases, January 2009
|
| DOI | 10.1371/journal.pntd.0000367 |
| Pubmed ID | |
| Authors |
Rebecca J. Traub, Julie Macaranas, Mathirut Mungthin, Saovanee Leelayoova, Thomas Cribb, K. Darwin Murrell, R. C. Andrew Thompson |
| Abstract |
Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. Epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. A PCR test capable of amplifying a segment of the internal transcribed spacer region of ribosomal DNA for the opisthorchiid and heterophyid flukes eggs taken directly from faeces was developed and evaluated in a rural community in central Thailand. The lowest quantity of DNA that could be amplified from individual adults of Opisthorchis viverrini, Clonorchis sinensis and Haplorchis taichui was estimated at 0.6 pg, 0.8 pg and 3 pg, respectively. The PCR was capable of detecting mixed infection with the aforementioned species of flukes under experimental conditions. A total of 11.6% of individuals in rural communities in Sanamchaikaet district, central Thailand, were positive for 'Opisthorchis-like' eggs in their faeces using conventional parasitological detection techniques. In comparison to microscopy, the PCR yielded a sensitivity and specificity of 71.0% and 76.7%, respectively. Analysis of the microscopy-positive PCR products revealed 64% and 23% of individuals to be infected with O. viverrini and C. sinensis, respectively. The remaining 13% (three individuals) were identified as eggs of Didymozoidae, presumably being passed mechanically in the faeces following the ingestion of infected fishes. An immediate finding of this study is the identification and first report of a C. sinensis-endemic community in central Thailand. This extends the known range of this liver fluke in Southeast Asia. The PCR developed herein provides an important tool for the specific identification of liver and intestinal fluke species for future epidemiological surveys. |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 3 | 5% |
| United Kingdom | 1 | 2% |
| Philippines | 1 | 2% |
| France | 1 | 2% |
| Unknown | 58 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 25% |
| Student > Master | 6 | 9% |
| Student > Bachelor | 5 | 8% |
| Student > Ph. D. Student | 5 | 8% |
| Lecturer | 4 | 6% |
| Other | 10 | 16% |
| Unknown | 18 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 20% |
| Biochemistry, Genetics and Molecular Biology | 10 | 16% |
| Veterinary Science and Veterinary Medicine | 7 | 11% |
| Medicine and Dentistry | 6 | 9% |
| Nursing and Health Professions | 2 | 3% |
| Other | 7 | 11% |
| Unknown | 19 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2017.
All research outputs
#8,544,090
of 25,394,764 outputs
Outputs from PLoS Neglected Tropical Diseases
#5,118
of 9,380 outputs
Outputs of similar age
#53,569
of 184,770 outputs
Outputs of similar age from PLoS Neglected Tropical Diseases
#18
of 36 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,380 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.0. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 184,770 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.