| Title |
In vitro antileishmanial and cytotoxicity activities of essential oils from Haplophyllum tuberculatum A. Juss leaves, stems and aerial parts
|
|---|---|
| Published in |
BMC Complementary Medicine and Therapies, February 2018
|
| DOI | 10.1186/s12906-018-2128-6 |
| Pubmed ID | |
| Authors |
Assia Hamdi, Joanne Bero, Claire Beaufay, Guido Flamini, Zohra Marzouk, Yvan Vander Heyden, Joelle Quetin-Leclercq |
| Abstract |
Plants used for traditional medicine produce diverse and complex secondary metabolites exhibiting various medicinal properties. The medicinal plant Haplophyllum tuberculatum is used by native people against malaria and parasitic infections. In this study and in order to contribute for the search of new natural drugs for leishmaniasis, the essential oils of H. tuberculatum leaves, stems and aerial parts (leaves+stems) collected in two different periods, 2013 and 2015, and their components by GC/FID and GC/MS analyses were investigated. Those collected in 2013 were also re-analyzed two years later. The extracted oils were screened in vitro for anti-leishmanial activity on Leishmania mexicana mexicana (L.m.m.) promastigotes and cytotoxicity on the Chinese Hamster Ovary (CHO) cell line. Limonene (1.5 - 8%), its isomers (R- (+)-limonene and S-(-)-limonene), linalool and octanol were also tested. Results showed that the chemical composition varied according to the year of collection. Though major compounds remain almost the same, qualitative and quantitative variations in the composition of the EOs can be observed between the two years of collection, with some minor compounds identified only in one type of samples. Variation in the composition were also observed in the re-analyzed volatile oils, showing stability concerns. The essential oils and R-(+)-limonene showed moderate anti-leishmanial activity. Their IC50range from 6.48 to 50.28 μg/ml. Cytotoxicity assays for theses volatile extracts, R- (+)-limonene and S- (-)-limonene on CHO cells showed relatively potent cytotoxicity with a selectivity index <10. Their CC50range from 27.79 to 82.56 μg/ml. The findings of the present study demonstrated that H. tuberculatum might not be considered as a natural source for production of new anti-leishmanial agents without further analyzing its eventual in vivo toxicity as well as that of major pure compounds. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 16% |
| Student > Master | 8 | 11% |
| Student > Bachelor | 5 | 7% |
| Researcher | 4 | 5% |
| Professor > Associate Professor | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 34 | 46% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 9 | 12% |
| Chemistry | 4 | 5% |
| Immunology and Microbiology | 4 | 5% |
| Medicine and Dentistry | 3 | 4% |
| Other | 7 | 9% |
| Unknown | 37 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#14,376,243
of 23,023,224 outputs
Outputs from BMC Complementary Medicine and Therapies
#1,697
of 3,644 outputs
Outputs of similar age
#244,586
of 446,257 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#51
of 109 outputs
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