| Title |
Engineering Corynebacterium glutamicum for isobutanol production
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2010
|
| DOI | 10.1007/s00253-010-2522-6 |
| Pubmed ID | |
| Authors |
Kevin Michael Smith, Kwang-Myung Cho, James C. Liao |
| Abstract |
The production of isobutanol in microorganisms has recently been achieved by harnessing the highly active 2-keto acid pathways. Since these 2-keto acids are precursors of amino acids, we aimed to construct an isobutanol production platform in Corynebacterium glutamicum, a well-known amino-acid-producing microorganism. Analysis of this host's sensitivity to isobutanol toxicity revealed that C. glutamicum shows an increased tolerance to isobutanol relative to Escherichia coli. Overexpression of alsS of Bacillus subtilis, ilvC and ilvD of C. glutamicum, kivd of Lactococcus lactis, and a native alcohol dehydrogenase, adhA, led to the production of 2.6 g/L isobutanol and 0.4 g/L 3-methyl-1-butanol in 48 h. In addition, other higher chain alcohols such as 1-propanol, 2-methyl-1-butanol, 1-butanol, and 2-phenylethanol were also detected as byproducts. Using longer-term batch cultures, isobutanol titers reached 4.0 g/L after 96 h with wild-type C. glutamicum as a host. Upon the inactivation of several genes to direct more carbon through the isobutanol pathway, we increased production by approximately 25% to 4.9 g/L isobutanol in a pycldh background. These results show promise in engineering C. glutamicum for higher chain alcohol production using the 2-keto acid pathways. |
Mendeley readers
The data shown below were compiled from readership statistics for 267 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 1% |
| Colombia | 1 | <1% |
| Germany | 1 | <1% |
| Thailand | 1 | <1% |
| Unknown | 260 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 72 | 27% |
| Researcher | 62 | 23% |
| Student > Master | 38 | 14% |
| Student > Bachelor | 18 | 7% |
| Professor > Associate Professor | 9 | 3% |
| Other | 33 | 12% |
| Unknown | 35 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 109 | 41% |
| Biochemistry, Genetics and Molecular Biology | 51 | 19% |
| Engineering | 26 | 10% |
| Chemistry | 17 | 6% |
| Chemical Engineering | 8 | 3% |
| Other | 16 | 6% |
| Unknown | 40 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2022.
All research outputs
#8,022,830
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,748
of 8,034 outputs
Outputs of similar age
#36,153
of 98,023 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#42
of 77 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
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We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one is in the 2nd percentile – i.e., 2% of its contemporaries scored the same or lower than it.