| Title |
Sequestration of Host Plant Glucosinolates in the Defensive Hemolymph of the Sawfly Athalia rosae
|
|---|---|
| Published in |
Journal of Chemical Ecology, December 2001
|
| DOI | 10.1023/a:1013631616141 |
| Pubmed ID | |
| Authors |
Caroline Müller, Niels Agerbirk, Carl Erik Olsen, Jean-Luc Boevé, Urs Schaffner, Paul M. Brakefield |
| Abstract |
Interactions between insects and glucosinolate-containing plant species have been investigated for a long time. Although the glucosinolate-myrosinase system is believed to act as a defense mechanism against generalist herbivores and fungi, several specialist insects use these secondary metabolites for host plant finding and acceptance and can handle them physiologically. However, sequestration of glucosinolates in specialist herbivores has been less well studied. Larvae of the tumip sawfly Athalia rosae feed on several glucosinolate-containing plant species. When larvae are disturbed by antagonists, they release one or more small droplets of hemolymph from their integument. This "reflex bleeding" is used as a defense mechanism. Specific glucosinolate analysis, by conversion to desulfoglucosinolates and analysis of these by high-performance liquid chromatography coupled to diode array UV spectroscopy and mass spectrometry, revealed that larvae incorporate and concentrate the plant's characteristic glucosinolates from their hosts. Extracts of larvae that were reared on Sinapis alba contained sinalbin, even when the larvae were first starved for 22 hr and, thus, had empty guts. Hemolymph was analyzed from larvae that were reared on either S. alba, Brassica nigra, or Barbarea stricta. Leaves were analyzed from the same plants the larvae had fed on. Sinalbin (from S. alba), sinigrin (B. nigra), or glucobarbarin and glucobrassicin (B. stricta) were present in leaves in concentrations less than 1 micromol/g fresh weight, while the same glucosinolates could be detected in the larvae's hemolymph in concentrations between 10 and 31 micromol/g fresh weight, except that glucobrassicin was present only as a trace. In larval feces, only trace amounts of glucosinolates (sinalbin and sinigrin) could be detected. The glucosinolates were likewise found in freshly emerged adults, showing that the sequestered phytochemicals were transferred through the pupal stage. |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Germany | 1 | 1% |
| Malaysia | 1 | 1% |
| Portugal | 1 | 1% |
| Brazil | 1 | 1% |
| France | 1 | 1% |
| Japan | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 81 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 21% |
| Student > Ph. D. Student | 17 | 19% |
| Student > Master | 12 | 13% |
| Student > Doctoral Student | 6 | 7% |
| Student > Bachelor | 6 | 7% |
| Other | 17 | 19% |
| Unknown | 13 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 51 | 57% |
| Environmental Science | 9 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 6% |
| Chemistry | 5 | 6% |
| Earth and Planetary Sciences | 2 | 2% |
| Other | 2 | 2% |
| Unknown | 16 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 June 2019.
All research outputs
#2,632,458
of 25,371,288 outputs
Outputs from Journal of Chemical Ecology
#123
of 2,151 outputs
Outputs of similar age
#4,964
of 132,007 outputs
Outputs of similar age from Journal of Chemical Ecology
#2
of 8 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 132,007 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.