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Characterization and Gene Expression Profiling of Five New Human Embryonic Stem Cell Lines Derived in Taiwan

Overview of attention for article published in Stem Cells & Development, August 2006
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Title
Characterization and Gene Expression Profiling of Five New Human Embryonic Stem Cell Lines Derived in Taiwan
Published in
Stem Cells & Development, August 2006
DOI 10.1089/scd.2006.15.532
Pubmed ID
Authors

Steven Shoei-Lung Li, Yung-Hsien Liu, Chao-Neng Tseng, Tung-Liang Chung, Tzi-Yi Lee, Sher Singh

Abstract

Many human embryonic stem cell (hESC) lines have been reported, but only a few of them have been fully characterized. In this report, five new hESC lines were derived from 32 discarded blastocysts in Taiwan, and these lines were continuously cultured on mitotically inactivated mouse embryonic fibroblast (MEF) feeder layer in the hESC medium for more than 44 passages and underwent freezing/thawing processes. All five hESC lines expressed characteristic undifferentiated hESC markers, such as SSEA-4, TRA-1-81, alkaline phosphatase, TERT, and the transcription factors POU5F1 (OCT4) and NANOG. hESC lines T1 and T3 possess normal female karyotypes, whereas lines T4 and T5 are normal male, but line T2 is male trisomy 12 (47XY,+12). hESC lines T1, T2, T3, and T5 were able to produce teratomas in severe combined immunodeficient (SCID) mice, and line T4 could only form embryoid bodies (EBs) in vitro. Global gene expression profiles of these five newly derived hESC lines were analyzed using the Affymetrix human genome U133 plus 2.0 GeneChip. The results showed that 4,145 transcripts, including 19% of unknown functions, were detected in all five hESC lines. Comparison of the 4,145 genes commonly expressed in the five hESC lines with those genes expressed in teratomas produced by the hESC line T1 and placenta revealed 40 genes exclusively expressed in all five hESC lines. These 40 genes include the previously reported stemness genes, such as POU5F1 (OCT4), NANOG, TDGF1 (CRIPTO), SALL4, LECT1, and BUB1 responsible for self-renewal and pluripotent differentiation. The global gene expression analysis also indicated that the transforming growth factor-beta (TGF-beta)/activin branch components inhibin BC, ACVR2A, ACVR1 (ALK2), TGFBR1 (ALK5), and SMAD2 were found to be highly expressed in undifferentiated states of these five hESC lines and decreased upon differentiation. In short, the hESC nature of these five hESC lines is supported by the undifferentiated state, extensive renewal capacity, and pluripotency, including the ability to form teratomas and/or EBs. These cell lines will be useful for human embryonic stem cell biology and drug development.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 45%
Lecturer > Senior Lecturer 1 9%
Student > Doctoral Student 1 9%
Other 1 9%
Student > Ph. D. Student 1 9%
Other 1 9%
Unknown 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Biochemistry, Genetics and Molecular Biology 2 18%
Philosophy 1 9%
Physics and Astronomy 1 9%
Medicine and Dentistry 1 9%
Other 0 0%
Unknown 1 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 May 2011.
All research outputs
#8,535,684
of 25,377,790 outputs
Outputs from Stem Cells & Development
#770
of 2,037 outputs
Outputs of similar age
#30,666
of 90,183 outputs
Outputs of similar age from Stem Cells & Development
#2
of 7 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,037 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 90,183 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.