Title |
Fluorescent cell-based sensing approaches for toxicity testing
|
---|---|
Published in |
Analytical & Bioanalytical Chemistry, April 2010
|
DOI | 10.1007/s00216-010-3651-6 |
Pubmed ID | |
Authors |
Michael Fritzsche, Carl-Fredrik Mandenius |
Abstract |
Fluorimetric cell-based sensing methods have attracted increasing interest in toxicity testing of pharmaceuticals, pathogens, environmental pollutants, and other chemicals. The objective of this review is to summarise the variety of approaches reported up to now and to present recent developments in this area. The different approaches are described in relation to their underlying mechanism and, especially, to the role of the fluorophore involved. The methods discussed include the use of fluorescent or fluorogenic indicators, fluorescence-based testing for membrane integrity, approaches based on fluorescence labelling, inducible fluorescent protein expression, and analysis of cellular autofluorescence. Several of these approaches have been shown to be advantageous in comparison with non-fluorescence methods and have potential in high-throughput screening, for example in drug discovery and safety pharmacology. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 3% |
Australia | 1 | 1% |
Unknown | 69 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 20 | 28% |
Student > Master | 12 | 17% |
Researcher | 10 | 14% |
Student > Bachelor | 8 | 11% |
Student > Doctoral Student | 3 | 4% |
Other | 6 | 8% |
Unknown | 13 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 14 | 19% |
Engineering | 10 | 14% |
Biochemistry, Genetics and Molecular Biology | 9 | 13% |
Chemistry | 8 | 11% |
Medicine and Dentistry | 5 | 7% |
Other | 11 | 15% |
Unknown | 15 | 21% |