Title |
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
|
---|---|
Published in |
Molecular Genetics and Genomics, November 1984
|
DOI | 10.1007/bf00330984 |
Pubmed ID | |
Authors |
Jef D. Boeke, Francois La Croute, Gerald R. Fink |
Abstract |
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 606 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 5 | <1% |
United States | 4 | <1% |
Netherlands | 3 | <1% |
United Kingdom | 3 | <1% |
Germany | 2 | <1% |
Japan | 2 | <1% |
Spain | 2 | <1% |
Albania | 1 | <1% |
South Africa | 1 | <1% |
Other | 2 | <1% |
Unknown | 581 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 155 | 26% |
Researcher | 96 | 16% |
Student > Bachelor | 92 | 15% |
Student > Master | 82 | 14% |
Student > Doctoral Student | 23 | 4% |
Other | 78 | 13% |
Unknown | 80 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 270 | 45% |
Biochemistry, Genetics and Molecular Biology | 175 | 29% |
Immunology and Microbiology | 14 | 2% |
Chemistry | 12 | 2% |
Engineering | 10 | 2% |
Other | 33 | 5% |
Unknown | 92 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2023.
All research outputs
#2,759,744
of 25,373,627 outputs
Outputs from Molecular Genetics and Genomics
#37
of 3,318 outputs
Outputs of similar age
#335
of 9,110 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 21 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,318 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.