Title |
An efficient 3D NMR technique for correlating the proton and15N backbone amide resonances with the α-carbon of the preceding residue in uniformly15N/13C enriched proteins
|
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Published in |
Journal of Biomolecular NMR, May 1991
|
DOI | 10.1007/bf01874573 |
Pubmed ID | |
Authors |
Ad Bax, Mitsuhiko Ikura |
Abstract |
A 3D NMR technique is described which correlates the amide proton and nitrogen resonances of an amino acid residue with the C alpha chemical shift of its preceding residue. The technique uses a relay mechanism, transferring magnetization from 15N to 13C alpha via the intervening carbonyl nucleus. This method for obtaining sequential connectivity is less sensitive to large line widths than the alternative HNCA experiment. The technique is demonstrated for the protein calmodulin, complexed with a 26 amino acid fragment of skeletal muscle myosin light chain kinase. |
Mendeley readers
The data shown below were compiled from readership statistics for 162 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
India | 1 | <1% |
United Kingdom | 1 | <1% |
Belgium | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Poland | 1 | <1% |
Unknown | 155 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 47 | 29% |
Researcher | 28 | 17% |
Student > Master | 15 | 9% |
Student > Bachelor | 14 | 9% |
Student > Doctoral Student | 9 | 6% |
Other | 24 | 15% |
Unknown | 25 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 48 | 30% |
Biochemistry, Genetics and Molecular Biology | 39 | 24% |
Agricultural and Biological Sciences | 32 | 20% |
Medicine and Dentistry | 3 | 2% |
Physics and Astronomy | 3 | 2% |
Other | 10 | 6% |
Unknown | 27 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2017.
All research outputs
#3,272,020
of 22,786,087 outputs
Outputs from Journal of Biomolecular NMR
#36
of 615 outputs
Outputs of similar age
#916
of 17,623 outputs
Outputs of similar age from Journal of Biomolecular NMR
#1
of 3 outputs
Altmetric has tracked 22,786,087 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 615 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done particularly well, scoring higher than 93% of its peers.
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We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them