| Title |
LexA-binding sequences in Gram-positive and cyanobacteria are closely related
|
|---|---|
| Published in |
Molecular Genetics and Genomics, December 2003
|
| DOI | 10.1007/s00438-003-0952-x |
| Pubmed ID | |
| Authors |
G. Mazón, J. M. Lucena, S. Campoy, A. R. Fernández de Henestrosa, P. Candau, J. Barbé |
| Abstract |
The lexA gene of the cyanobacterium Anabaena sp. strain PCC7120 has been cloned by PCR amplification with primers designed after TBLASTN analysis of its genome sequence using the Escherichia coli LexA sequence as a probe. After over-expression in E. coli and subsequent purification, footprinting experiments demonstrated that the Anabaena LexA protein binds to the sequence TAGTACTAATGTTCTA, which is found upstream of its own coding gene. Directed mutagenesis and sequence comparison of promoters of other Anabaena genes, as well as those of several cyanobacteria, allowed us to define the motif RGTACNNNDGTWCB as the LexA box in this bacterial phylum. Substitution of a single nucleotide in this motif present in the Anabena lexA promoter is sufficient to enable it to bind the Bacillus subtilis LexA protein. These data indicate that Cyanobacteria and Gram-positive bacteria are phylogenetically closely related. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Unknown | 29 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 30% |
| Researcher | 5 | 17% |
| Professor > Associate Professor | 4 | 13% |
| Student > Bachelor | 2 | 7% |
| Student > Doctoral Student | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 60% |
| Biochemistry, Genetics and Molecular Biology | 7 | 23% |
| Engineering | 2 | 7% |
| Immunology and Microbiology | 1 | 3% |
| Unknown | 2 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2021.
All research outputs
#8,535,684
of 25,374,917 outputs
Outputs from Molecular Genetics and Genomics
#920
of 3,319 outputs
Outputs of similar age
#36,141
of 142,790 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 8 outputs
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