Title |
Chicken anaemia virus evades host immune responses in transformed lymphocytes
|
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Published in |
Journal of General Virology, March 2018
|
DOI | 10.1099/jgv.0.001011 |
Pubmed ID | |
Authors |
Efstathios S Giotis, Alistair Scott, Lisa Rothwell, Tuanjun Hu, Richard Talbot, Daniel Todd, David W Burt, Elizabeth J Glass, Pete Kaiser |
Abstract |
Chicken anaemia virus (CAV) is a lymphotropic virus that causes anaemia and immunosuppression in chickens. Previously, we proposed that CAV evades host antiviral responses in vivo by disrupting T-cell signalling, but the precise cellular targets and modes of action remain elusive. In this study, we examined gene expression in Marek's disease virus-transformed chicken T-cell line MSB-1 after infection with CAV using both a custom 5K immune-focused microarray and quantitative real-time PCR at 24, 48 and 72 h post-infection. The data demonstrate an intricate equilibrium between CAV and the host gene expression, displaying subtle but significant modulation of transcripts involved in the T-cell, inflammation and NF-κB signalling cascades. CAV efficiently blocked the induction of type-I interferons and interferon-stimulated genes at 72 h. The cell expression pattern implies that CAV subverts host antiviral responses and that the transformed environment of MSB-1 cells offers an opportunistic advantage for virus growth. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 29% |
Student > Bachelor | 3 | 21% |
Professor | 2 | 14% |
Student > Doctoral Student | 1 | 7% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 36% |
Agricultural and Biological Sciences | 2 | 14% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Medicine and Dentistry | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 29% |