Title |
Human Chromosomal Fragile Site FRA16B Is an Amplified AT-Rich Minisatellite Repeat
|
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Published in |
Cell, February 1997
|
DOI | 10.1016/s0092-8674(00)81875-9 |
Pubmed ID | |
Authors |
Sui Yu, Marie Mangelsdorf, Duncan Hewett, Lynne Hobson, Elizabeth Baker, Helen J Eyre, Naras Lapsys, Denis Le Paslier, Norman A Doggett, Grant R Sutherland, Robert I Richards |
Abstract |
Fragile sites are nonstaining gaps in chromosomes induced by specific tissue culture conditions. They vary both in population frequency and in the culture conditions required for induction. Folate-sensitive fragile sites are due to expansion of p(CCG)n trinucleotide repeats; however, the relationship between sequence composition and the chemistry of induction of fragile sites is unclear. To clarify this relationship, the distamycin A-sensitive fragile site FRA16B was isolated by positional cloning and found to be an expanded 33 bp AT-rich minisatellite repeat, p(ATATA TTATATATTATATCTAATAATATATC/ATA)n (consistent with DNA sequence binding preferences of chemicals that induce its cytogenetic expression). Therefore the mutation mechanism associated with trinucleotide repeats is also a property of minisatellite repeats (variable number tandem repeats). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 5% |
Russia | 1 | 3% |
France | 1 | 3% |
Unknown | 33 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 24% |
Student > Ph. D. Student | 7 | 19% |
Professor > Associate Professor | 6 | 16% |
Professor | 3 | 8% |
Student > Doctoral Student | 2 | 5% |
Other | 5 | 14% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 49% |
Biochemistry, Genetics and Molecular Biology | 10 | 27% |
Medicine and Dentistry | 2 | 5% |
Arts and Humanities | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 1 | 3% |
Unknown | 4 | 11% |