| Title |
Requirements of Slm proteins for proper eisosome organization, endocytic trafficking and recycling in the yeast Saccharomyces cerevisiae
|
|---|---|
| Published in |
Proceedings: Plant Sciences, April 2011
|
| DOI | 10.1007/s12038-011-9018-0 |
| Pubmed ID | |
| Authors |
Chitra Kamble, Sandhya Jain, Erin Murphy, Kyoungtae Kim |
| Abstract |
Eisosomes are large immobile assemblies at the cortex of a cell under the membrane compartment of Can1 (MCC) in yeast. Slm1 has recently been identified as an MCC component that acts downstream of Mss4 in a pathway that regulates actin cytoskeleton organization in response to stress. In this study, we showed that inactivation of Slm proteins disrupts proper localization of the primary eisosome marker Pil1, providing evidence that Slm proteins play a role in eisosome organization. Furthermore, we found that slm ts mutant cells exhibit actin defects in both the ability to polarize cortical F-actin and the formation of cytoplasmic actin cables even at the permissive temperature (30 degrees C). We further demonstrated that the actin defect accounts for the slow traffic of FM4-64- labelled endosome in the cytoplasm, supporting the notion that intact actin is essential for endosome trafficking. However, our real-time microscopic analysis of Abp1-RFP revealed that the actin defect in slm ts cells was not accompanied by a noticeable defect in actin patch internalization during receptor-mediated endocytosis. In addition, we found that slm ts cells displayed impaired membrane recycling and that recycling occurred in an actin-independent manner. Our data provide evidence for the requirement of Slm proteins in eisosome organization and endosome trafficking and recycling. |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 30% |
| Researcher | 9 | 23% |
| Student > Bachelor | 5 | 13% |
| Student > Master | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 40% |
| Agricultural and Biological Sciences | 15 | 38% |
| Arts and Humanities | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Psychology | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 4 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2012.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Proceedings: Plant Sciences
#242
of 975 outputs
Outputs of similar age
#45,238
of 120,960 outputs
Outputs of similar age from Proceedings: Plant Sciences
#5
of 12 outputs
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