| Title |
Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study
|
|---|---|
| Published in |
Histochemistry and Cell Biology, October 2007
|
| DOI | 10.1007/s00418-007-0337-z |
| Pubmed ID | |
| Authors |
C. Csaki, U. Matis, A. Mobasheri, H. Ye, M. Shakibaei |
| Abstract |
Musculoskeletal diseases with osteochondrotic articular cartilage defects, such as osteoarthritis, are an increasing problem for humans and companion animals which necessitates the development of novel and improved therapeutic strategies. Canine mesenchymal stem cells (cMSCs) offer significant promise as a multipotent source for cell-based therapies and could form the basis for the differentiation and cultivation of tissue grafts to replace damaged tissue. However, no comprehensive analysis has been undertaken to characterize the ultrastructure of in vitro differentiated cMSCs. The main goal of this paper was to focus on cMSCs and to analyse their differentiation capacity. To achieve this aim, bone marrow cMSCs from three canine patients were isolated, expanded in monolayer culture and characterized with respect to their ability for osteogenic, adipogenic and chondrogenic differentiation capacities. cMSCs showed proliferative potential and were capable of osteogenic, adipogenic and chondrogenic differentiation. cMSCs treated with the osteogenic induction medium differentiated into osteoblasts, produced typical bone matrix components, beta1-integrins and upregulated the osteogenic specific transcription factor Cbfa-1. cMSCs treated with the adipogenic induction medium showed typical adipocyte morphology, produced adiponectin, collagen type I and beta1-integrins, and upregulated the adipogenic specific transcription factor PPAR-gamma. cMSCs treated with the chondrogenic induction medium exhibited a round to oval shape, produced a cartilage-specific extracellular matrix, beta1-integrins and upregulated the chondrogenic specific transcription factor Sox9. These results demonstrate, at the biochemical, morphological and ultrastructural levels, the multipotency of cMSCs and thus highlight their potential therapeutic value for cell-based tissue engineering. |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | <1% |
| Brazil | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Peru | 1 | <1% |
| Iran, Islamic Republic of | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 117 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 20 | 16% |
| Researcher | 19 | 15% |
| Student > Ph. D. Student | 19 | 15% |
| Student > Postgraduate | 11 | 9% |
| Professor | 10 | 8% |
| Other | 27 | 22% |
| Unknown | 18 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 31% |
| Medicine and Dentistry | 29 | 23% |
| Biochemistry, Genetics and Molecular Biology | 18 | 15% |
| Veterinary Science and Veterinary Medicine | 12 | 10% |
| Immunology and Microbiology | 2 | 2% |
| Other | 6 | 5% |
| Unknown | 19 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2019.
All research outputs
#2,945,405
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#40
of 926 outputs
Outputs of similar age
#7,309
of 74,157 outputs
Outputs of similar age from Histochemistry and Cell Biology
#1
of 5 outputs
Altmetric has tracked 24,217,893 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 926 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 74,157 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them