Title |
MicroRNA degradation by a conserved target RNA regulates animal behavior
|
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Published in |
Nature Structural & Molecular Biology, February 2018
|
DOI | 10.1038/s41594-018-0032-x |
Pubmed ID | |
Authors |
Angelo Bitetti, Allison C. Mallory, Elisabetta Golini, Claudia Carrieri, Héctor Carreño Gutiérrez, Emerald Perlas, Yuvia A. Pérez-Rico, Glauco P. Tocchini-Valentini, Anton J. Enright, William H. J. Norton, Silvia Mandillo, Dónal O’Carroll, Alena Shkumatava |
Abstract |
microRNAs (miRNAs) repress target transcripts through partial complementarity. By contrast, highly complementary miRNA-binding sites within viral and artificially engineered transcripts induce miRNA degradation in vitro and in cell lines. Here, we show that a genome-encoded transcript harboring a near-perfect and deeply conserved miRNA-binding site for miR-29 controls zebrafish and mouse behavior. This transcript originated in basal vertebrates as a long noncoding RNA (lncRNA) and evolved to the protein-coding gene NREP in mammals, where the miR-29-binding site is located within the 3' UTR. We show that the near-perfect miRNA site selectively triggers miR-29b destabilization through 3' trimming and restricts its spatial expression in the cerebellum. Genetic disruption of the miR-29 site within mouse Nrep results in ectopic expression of cerebellar miR-29b and impaired coordination and motor learning. Thus, we demonstrate an endogenous target-RNA-directed miRNA degradation event and its requirement for animal behavior. |
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United Kingdom | 3 | 8% |
France | 2 | 5% |
Norway | 1 | 3% |
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Germany | 1 | 3% |
Egypt | 1 | 3% |
Other | 4 | 10% |
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Demographic breakdown
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Scientists | 16 | 41% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 169 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 38 | 22% |
Researcher | 35 | 21% |
Student > Master | 16 | 9% |
Student > Bachelor | 16 | 9% |
Student > Doctoral Student | 10 | 6% |
Other | 17 | 10% |
Unknown | 37 | 22% |
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Neuroscience | 14 | 8% |
Medicine and Dentistry | 6 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 2% |
Other | 8 | 5% |
Unknown | 42 | 25% |