| Title |
Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cultured with degraded collagen
|
|---|---|
| Published in |
Cell and Tissue Research, February 2003
|
| DOI | 10.1007/s00441-003-0702-8 |
| Pubmed ID | |
| Authors |
Steffen Oesser, Jürgen Seifert |
| Abstract |
The functional integrity of articular cartilage is dependent on the maintenance of the extracellular matrix (ECM), a process which is controlled by chondrocytes. The regulation of ECM biosynthesis is complex and a variety of substances have been found to influence chondrocyte metabolism. In the present study we have investigated the effect of degraded collagen on the formation of type II collagen by mature bovine chondrocytes in a cell culture model. The culture medium was supplemented with collagen hydrolysate (CH) and biosynthesis of type II collagen by chondrocytes was compared to control cells treated with native type I and type II collagen and a collagen-free protein hydrolysate. The quantification of type II collagen by means of an ELISA technique was confirmed by immunocytochemical detection as well as by the incorporation of (14)C-proline in the ECM after a 48 h incubation. Chondrocytes in the control group were maintained in the basal medium for 11 days. The presence of extracellular CH led to a dose-dependent increase in type II collagen secretion. However, native collagens as well as a collagen-free hydrolysate of wheat proteins failed to stimulate the production of type II collagen in chondrocytes. These results clearly indicate a stimulatory effect of degraded collagen on the type II collagen biosynthesis of chondrocytes and suggest a possible feedback mechanism for the regulation of collagen turnover in cartilage tissue. |
Mendeley readers
The data shown below were compiled from readership statistics for 121 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| Sweden | 1 | <1% |
| Unknown | 117 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 23 | 19% |
| Student > Bachelor | 19 | 16% |
| Student > Master | 12 | 10% |
| Other | 11 | 9% |
| Student > Ph. D. Student | 9 | 7% |
| Other | 18 | 15% |
| Unknown | 29 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 20 | 17% |
| Agricultural and Biological Sciences | 18 | 15% |
| Biochemistry, Genetics and Molecular Biology | 17 | 14% |
| Engineering | 8 | 7% |
| Nursing and Health Professions | 6 | 5% |
| Other | 16 | 13% |
| Unknown | 36 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2021.
All research outputs
#8,537,346
of 25,377,790 outputs
Outputs from Cell and Tissue Research
#545
of 2,233 outputs
Outputs of similar age
#20,976
of 62,121 outputs
Outputs of similar age from Cell and Tissue Research
#2
of 5 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,233 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.