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Attention Score in Context
| Title |
Sulfite:Cytochrome c Oxidoreductase fromThiobacillus novellus PURIFICATION, CHARACTERIZATION, AND MOLECULAR BIOLOGY OF A HETERODIMERIC MEMBER OF THE SULFITE OXIDASE FAMILY*
|
|---|---|
| Published in |
Journal of Biological Chemistry, May 2000
|
| DOI | 10.1074/jbc.275.18.13202 |
| Pubmed ID | |
| Authors |
Ulrike Kappler, Brian Bennett, Jörg Rethmeier, Günter Schwarz, Rainer Deutzmann, Alistair G. McEwan, Christiane Dahl |
| Abstract |
Direct oxidation of sulfite to sulfate occurs in various photo- and chemotrophic sulfur oxidizing microorganisms as the final step in the oxidation of reduced sulfur compounds and is catalyzed by sulfite:cytochrome c oxidoreductase (EC ). Here we show that the enzyme from Thiobacillus novellus is a periplasmically located alphabeta heterodimer, consisting of a 40.6-kDa subunit containing a molybdenum cofactor and an 8.8-kDa mono-heme cytochrome c(552) subunit (midpoint redox potential, E(m8.0) = +280 mV). The organic component of the molybdenum cofactor was identified as molybdopterin contained in a 1:1 ratio to the Mo content of the enzyme. Electron paramagnetic resonance spectroscopy revealed the presence of a sulfite-inducible Mo(V) signal characteristic of sulfite:acceptor oxidoreductases. However, pH-dependent changes in the electron paramagnetic resonance signal were not detected. Kinetic studies showed that the enzyme exhibits a ping-pong mechanism involving two reactive sites. K(m) values for sulfite and cytochrome c(550) were determined to be 27 and 4 micrometer, respectively; the enzyme was found to be reversibly inhibited by sulfate and various buffer ions. The sorAB genes, which encode the enzyme, appear to form an operon, which is preceded by a putative extracytoplasmic function-type promoter and contains a hairpin loop termination structure downstream of sorB. While SorA exhibits significant similarities to known sequences of eukaryotic and bacterial sulfite:acceptor oxidoreductases, SorB does not appear to be closely related to any known c-type cytochromes. |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 1 | 3% |
| Germany | 1 | 3% |
| Tunisia | 1 | 3% |
| Unknown | 36 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Researcher | 6 | 15% |
| Student > Bachelor | 5 | 13% |
| Student > Master | 3 | 8% |
| Professor > Associate Professor | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 10 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 31% |
| Agricultural and Biological Sciences | 10 | 26% |
| Engineering | 2 | 5% |
| Environmental Science | 2 | 5% |
| Chemical Engineering | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 10 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2010.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Journal of Biological Chemistry
#32,957
of 85,241 outputs
Outputs of similar age
#13,798
of 40,860 outputs
Outputs of similar age from Journal of Biological Chemistry
#278
of 725 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 85,241 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 40,860 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 725 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.