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Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii

Overview of attention for article published in PLOS ONE, February 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

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1 blog
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22 X users
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1 Wikipedia page

Citations

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63 Dimensions

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125 Mendeley
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Title
Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii
Published in
PLOS ONE, February 2018
DOI 10.1371/journal.pone.0185039
Pubmed ID
Authors

Yu Zhan, Christophe H. Marchand, Alexandre Maes, Adeline Mauries, Yi Sun, James S. Dhaliwal, James Uniacke, Simon Arragain, Heng Jiang, Nicholas D. Gold, Vincent J. J. Martin, Stéphane D. Lemaire, William Zerges

Abstract

Organelles are intracellular compartments which are themselves compartmentalized. Biogenic and metabolic processes are localized to specialized domains or microcompartments to enhance their efficiency and suppress deleterious side reactions. An example of intra-organellar compartmentalization is the pyrenoid in the chloroplasts of algae and hornworts. This microcompartment enhances the photosynthetic CO2-fixing activity of the Calvin-Benson cycle enzyme Rubisco, suppresses an energetically wasteful oxygenase activity of Rubisco, and mitigates limiting CO2 availability in aquatic environments. Hence, the pyrenoid is functionally analogous to the carboxysomes in cyanobacteria. However, a comprehensive analysis of pyrenoid functions based on its protein composition is lacking. Here we report a proteomic characterization of the pyrenoid in the green alga Chlamydomonas reinhardtii. Pyrenoid-enriched fractions were analyzed by quantitative mass spectrometry. Contaminant proteins were identified by parallel analyses of pyrenoid-deficient mutants. This pyrenoid proteome contains 190 proteins, many of which function in processes that are known or proposed to occur in pyrenoids: e.g. the carbon concentrating mechanism, starch metabolism or RNA metabolism and translation. Using radioisotope pulse labeling experiments, we show that pyrenoid-associated ribosomes could be engaged in the localized synthesis of the large subunit of Rubisco. New pyrenoid functions are supported by proteins in tetrapyrrole and chlorophyll synthesis, carotenoid metabolism or amino acid metabolism. Hence, our results support the long-standing hypothesis that the pyrenoid is a hub for metabolism. The 81 proteins of unknown function reveal candidates for new participants in these processes. Our results provide biochemical evidence of pyrenoid functions and a resource for future research on pyrenoids and their use to enhance agricultural plant productivity. Data are available via ProteomeXchange with identifier PXD004509.

X Demographics

X Demographics

The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 125 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 17%
Student > Ph. D. Student 15 12%
Researcher 15 12%
Student > Master 15 12%
Student > Doctoral Student 7 6%
Other 13 10%
Unknown 39 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 42 34%
Agricultural and Biological Sciences 33 26%
Chemistry 2 2%
Environmental Science 1 <1%
Business, Management and Accounting 1 <1%
Other 6 5%
Unknown 40 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 24. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2020.
All research outputs
#1,373,121
of 23,025,074 outputs
Outputs from PLOS ONE
#17,914
of 196,293 outputs
Outputs of similar age
#32,760
of 330,211 outputs
Outputs of similar age from PLOS ONE
#420
of 3,592 outputs
Altmetric has tracked 23,025,074 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 196,293 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.2. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,211 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 3,592 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.