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AMPK

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Cover of 'AMPK'

Table of Contents

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    Book Overview
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    Chapter 1 Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (α1β1γ1)
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    Chapter 2 Visualizing AMPK Drug Binding Sites Through Crystallization of Full-Length Phosphorylated α2β1γ1 Heterotrimer
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    Chapter 3 Biophysical Interactions of Direct AMPK Activators
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    Chapter 4 Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship
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    Chapter 5 Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK
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    Chapter 6 Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate-Binding Modules
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    Chapter 7 Bioinformatics Approach to Identify Novel AMPK Targets
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    Chapter 8 Studying AMPK in an Evolutionary Context
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    Chapter 9 AMPK Protein Interaction Analyses by Yeast Two-Hybrid
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    Chapter 10 Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells
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    Chapter 11 Knockdown of Human AMPK Using the CRISPR/Cas9 Genome-Editing System
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    Chapter 12 Compound C/Dorsomorphin: Its Use and Misuse as an AMPK Inhibitor
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    Chapter 13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
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    Chapter 14 Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in the Skeletal Muscle
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    Chapter 15 Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography
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    Chapter 16 Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation
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    Chapter 17 Cellular Application of Genetically Encoded Sensors and Impeders of AMPK
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    Chapter 18 Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
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    Chapter 19 Study of AMPK-Regulated Metabolic Fluxes in Neurons Using the Seahorse XFe Analyzer
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    Chapter 20 Investigating the Role of AMPK in Inflammation
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    Chapter 21 Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis
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    Chapter 22 Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake
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    Chapter 23 Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells
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    Chapter 24 Studying the Role of AMPK in Autophagy
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    Chapter 25 Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis
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    Chapter 26 Manipulation and Measurement of AMPK Activity in Pancreatic Islets
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    Chapter 27 Analyzing AMPK Function in the Hypothalamus
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    Chapter 28 Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins
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    Chapter 29 A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress
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    Chapter 30 Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis
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    Chapter 31 Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative Stress
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    Chapter 32 Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels
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    Chapter 33 Studying the Role of AMPK in Angiogenesis
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    Chapter 34 Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity
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    Chapter 35 Evaluating the Role of Host AMPK in Leishmania Burden
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    Chapter 36 Analysis of Transgenerational Phenotypes Following Acute Starvation in AMPK-Deficient C. elegans
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    Chapter 37 Human γ2-AMPK Mutations
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Title
AMPK
Published by
Humana Press, New York, NY, March 2018
DOI 10.1007/978-1-4939-7598-3
ISBNs
978-1-4939-7597-6, 978-1-4939-7598-3
Editors

Dietbert Neumann, Benoit Viollet

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