| Title |
Biochemical characterization of a thermostable endomannanase/endoglucanase from Dictyoglomus turgidum
|
|---|---|
| Published in |
Extremophiles, November 2017
|
| DOI | 10.1007/s00792-017-0983-6 |
| Pubmed ID | |
| Authors |
Francesca Anna Fusco, Raffaele Ronca, Gabriella Fiorentino, Emilia Pedone, Patrizia Contursi, Simonetta Bartolucci, Danila Limauro |
| Abstract |
Dictyoglomus turgidum is a hyperthermophilic, anaerobic, gram-negative bacterium that shows an array of putative glycoside hydrolases (GHs) encoded by its genome, a feature that makes this microorganism very interesting for biotechnological applications. The aim of this work is the characterization of a hyperthermophilic GH5, Dtur_0671, of D. turgidum, annotated as endoglucanase and herein named DturCelB in agreement to DturCelA, which was previously characterized. The synthetic gene was expressed in Escherichia coli. The purified recombinant enzyme is active as a monomer (40 kDa) and CD structural studies showed a conserved α/β structure at different temperatures (25 and 70 °C) and high thermoresistance (Tm of 88 °C). Interestingly, the enzyme showed high endo-β-1,4-mannanase activity vs various mannans, but low endo-β-1,4 glucanase activity towards carboxymethylcellulose. The K M and V max of DturCelB were determined for both glucomannan and CMC: they were 4.70 mg/ml and 473.1 μmol/min mg and 1.83 mg/ml and 1.349 μmol/min mg, respectively. Its optimal activity towards temperature and pH resulted to be 70 °C and pH 5.4, respectively. Further characterization highlighted good thermal stability (~ 50% of enzymatic activity after 2 h at 70 °C) and pH stability over a broad range (> 90% of activity after 1 h in buffer, ranging pH 5-9); resistance to chemicals was also observed. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 51 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 14% |
| Student > Master | 6 | 12% |
| Student > Bachelor | 5 | 10% |
| Researcher | 4 | 8% |
| Professor > Associate Professor | 3 | 6% |
| Other | 7 | 14% |
| Unknown | 19 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 31% |
| Agricultural and Biological Sciences | 4 | 8% |
| Environmental Science | 3 | 6% |
| Chemical Engineering | 1 | 2% |
| Computer Science | 1 | 2% |
| Other | 4 | 8% |
| Unknown | 22 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2018.
All research outputs
#15,493,741
of 23,025,074 outputs
Outputs from Extremophiles
#544
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Outputs of similar age
#265,437
of 438,242 outputs
Outputs of similar age from Extremophiles
#10
of 18 outputs
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