Title |
Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis
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Published in |
Nature Communications, February 2018
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DOI | 10.1038/s41467-018-03131-0 |
Pubmed ID | |
Authors |
Ladan Khodaparast, Laleh Khodaparast, Rodrigo Gallardo, Nikolaos N. Louros, Emiel Michiels, Reshmi Ramakrishnan, Meine Ramakers, Filip Claes, Lydia Young, Mohammad Shahrooei, Hannah Wilkinson, Matyas Desager, Wubishet Mengistu Tadesse, K. Peter R. Nilsson, Per Hammarström, Abram Aertsen, Sebastien Carpentier, Johan Van Eldere, Frederic Rousseau, Joost Schymkowitz |
Abstract |
Aggregation is a sequence-specific process, nucleated by short aggregation-prone regions (APRs) that can be exploited to induce aggregation of proteins containing the same APR. Here, we find that most APRs are unique within a proteome, but that a small minority of APRs occur in many proteins. When aggregation is nucleated in bacteria by such frequently occurring APRs, it leads to massive and lethal inclusion body formation containing a large number of proteins. Buildup of bacterial resistance against these peptides is slow. In addition, the approach is effective against drug-resistant clinical isolates of Escherichia coli and Acinetobacter baumannii, reducing bacterial load in a murine bladder infection model. Our results indicate that redundant APRs are weak points of bacterial protein homeostasis and that targeting these may be an attractive antibacterial strategy. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 3 | 23% |
Belgium | 2 | 15% |
Canada | 2 | 15% |
United States | 1 | 8% |
Australia | 1 | 8% |
Unknown | 4 | 31% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 62% |
Scientists | 3 | 23% |
Science communicators (journalists, bloggers, editors) | 2 | 15% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 133 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 29 | 22% |
Researcher | 21 | 16% |
Student > Master | 17 | 13% |
Student > Bachelor | 15 | 11% |
Other | 7 | 5% |
Other | 20 | 15% |
Unknown | 24 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 53 | 40% |
Agricultural and Biological Sciences | 16 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 10 | 8% |
Immunology and Microbiology | 8 | 6% |
Chemistry | 6 | 5% |
Other | 15 | 11% |
Unknown | 25 | 19% |